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Dissemination of Bt cotton in cotton growing belt of Pakistan

机译：Bt棉在巴基斯坦棉花种植区的传播

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Background: Adoptability of biotech crops has climbed up dramatically in the world from1.7 million hectares in its introductory period in 1996 to 170 million hectare in 2012. Areaplanted to biotech crop increased to over one-fourth of the world total in 2005-06. The datacollected also demonstrates that developing countries like Pakistan are making tremendousdevelopment in production of Biotech crops especially cotton.Methodology: Random samples have been collected and received from different cottongrowing areas of Pakistan from 2007 to date for Bt gene confirmation and expression.Genomic DNA was isolated and analyzed through internal reference primers from SadI geneof cotton genome. Samples were analyzed by PCR for detection of Bt genes including CEMBCry1Ac+2A double Bt gene. ELISA was done for confirmation of Bt protein by usingEnvirologix Quantiplate ELISA kit Cry1Ab/Ac Cat # AP003 and Quantiplate ELISA kitCry2A Cat # AP005 according to manufacturer instruction.Results: Development of Bt Cotton in Pakistan is as old as in advanced countries of theworld. Pakistan has become the fourth country of the world for production of indigenous Btcotton by utilizing their local cotton varieties for genetic modifications. The support ofvarious documentary proofs like research articles, patents, projects, Ph. D and M.Phil studiesgenerated by Centre of Excellence in Molecular Biology (CEMB) greatly strengthen the dataof Bt cotton development in Pakistan. Pakistan Central Cotton Committee (PCCC)declaredlocally developed Bt cotton as the best performing of all over Pakistan among otherinternational product.Conclusion: Progress made in development of indigenous Bt cotton variety by CEMBPakistan and their excellent performance in field determined their increased adoptability ratioin farmers. The formal approval process which is going to be completed in near future willopen the doors for farmers and breeders to utilize this material for better economy ofPakistan.
机译：背景：全球转基因作物的可利用性已从1996年开始的170万公顷急剧增加到2012年的1.7亿公顷。转基因作物的播种面积在2005-06年度增加到世界总面积的四分之一以上。收集的数据还表明，巴基斯坦等发展中国家在转基因作物特别是棉花的生产方面正在取得巨大发展。方法：自2007年至今，已从巴基斯坦不同棉花种植地区收集并接收了随机样本用于Bt基因确认和表达。并通过棉花基因组SadI基因的内部参考引物进行分析。通过PCR分析样品以检测包括CEMBCry1Ac + 2A双Bt基因的Bt基因。根据生产商的说明，使用Envirologix Quantiplate ELISA试剂盒Cry1Ab / Ac Cat＃AP003和Quantiplate ELISA试剂盒Cry2A Cat＃AP005进行Bt蛋白的确认。结果：巴基斯坦Bt Cotton的开发与世界先进国家一样古老。巴基斯坦通过利用其本地棉花品种进行基因改造，已成为世界上第四大生产原生Btcotton的国家。由卓越分子生物学中心（CEMB）生成的各种文献证明，如研究文章，专利，项目，博士学位和M.Phil研究的支持，大大加强了巴基斯坦Bt棉花发展的数据。巴基斯坦中央棉花委员会（PCCC）宣布，在其他国际产品中，巴基斯坦本地种植的Bt棉花表现最佳。结论：CEMBP巴基斯坦在开发本地Bt棉花品种方面取得的进展以及其出色的表现决定了其在农民中的采用率提高。正式批准程序将在不久的将来完成，这将为农民和育种者利用这种材料改善巴基斯坦的经济打开大门。

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