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首页> 外文期刊>Aquatic Biology >Multixenobiotic resistance in coelomocytes from three echinoderm species
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Multixenobiotic resistance in coelomocytes from three echinoderm species

机译：三种棘皮动物的异种细胞对多异生物素的耐药性

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ABSTRACT: Multixenobiotic resistance (MXR) proteins are known to be present in most living organisms, but only a few studies have been conducted on echinoderms and especially on their circulating cells, the coelomocytes. The objective of the present study was to investigate the presence of MXR activity in coelomocytes of the sea urchin Strongylocentrotus droebachiensis, the sea star Lept­asterias polaris and the sea cucumber Cucumaria frondosa. Cells were exposed to fluorescent substrates (1 µM Rhodamine B [RB] or 0.5 µM calcein-AM [CAM]), with or without inhibitors (50 µM verapamil [Ver], 5 µM cyclosporin-A [CsA] and 5 µM MK571 [MK]), and single-cell fluorescence was measured by flow cytometry. The combinations RB + CsA and RB + Ver induced a fluorescence in­crease in S. droebachiensis and L. polaris coelomocytes, as well as in S. droebachiensis vibratile cells. The combination RB + MK induced a fluorescence increase in S. droebachiensis and C. frondosa coelomocytes. Finally, the combination CAM + MK induced a fluorescence diminution in L. polaris coelomocytes and S. droebachiensis vibratile cells. This difference in fluorescence incorporation indicated an MXR-like activity in coelomocytes, probably due to the presence of a P-glycoprotein (Pgp) and a multidrug resistance-associated protein (MRP)-like transporter. Western blot analysis was also carried out (Ab C219 and Ab C9) in order to detect potential MXR proteins using anti-MXR anti­bodies. Both Pgp and MRP were detected, but could not be further discriminated. MXR activity was clearly demonstrated in coelomocytes of S. droebachiensis, L. polaris and C. frondosa, although the identity of proteins responsible for this activity needs to be confirmed.
机译：摘要：已知大多数生物体中都存在多异源抗药性（MXR）蛋白，但对棘皮动物，尤其是其循环细胞，即成鞘细胞，仅进行了很少的研究。本研究的目的是调查海胆 Strongylocentrotus droebachiensis ，海星北极偏花鱼和海参黄瓜frondosa 。将细胞暴露于有或没有抑制剂（50 µM维拉帕米[Ver]，5 µM环孢菌素A [CsA]和5 µM MK571 [ MK]），并通过流式细胞仪测量单细胞荧光。 RB + CsA和RB + Ver的组合诱导i的荧光增加。 droebachiensis 和 L。北极星的内皮细胞，以及 S droebachiensis振动细胞。 RB + MK的组合引起iS的荧光增加。 droebachiensis 和 C。 frondosa 淋巴细胞。最后，CAM + MK的组合诱导L的荧光减弱。北极星皮层细胞和 S。 droebachiensis 振动细胞。荧光掺入的这种差异表明在内皮细胞中存在MXR样活性，这可能是由于P糖蛋白（Pgp）和多药耐药相关蛋白（MRP）样转运蛋白的存在。为了使用抗MXR抗体检测潜在的MXR蛋白，还进行了蛋白质印迹分析（Ab C219和Ab C9）。 Pgp和MRP均被检测到，但无法进一步区分。 MXR活性在iS的粒细胞中清楚地证明。 droebachiensis ， L。北极星和 C。 frondosa ，尽管需要确认负责此活动的蛋白质的身份。

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