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首页> 外文期刊>Applied and environmental soil science >Nickel in Soil Modifies Sensitivity to Diazinon Measured by the Activity of Acetylcholinesterase, Catalase, and Glutathione S-Transferase in EarthwormEisenia fetida
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Nickel in Soil Modifies Sensitivity to Diazinon Measured by the Activity of Acetylcholinesterase, Catalase, and Glutathione S-Transferase in EarthwormEisenia fetida

机译：通过Earth中乙酰胆碱酯酶，过氧化氢酶和谷胱甘肽S-转移酶的活性测定，土壤中的镍改变了对二嗪农的敏感性

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Nickel in typical soils is present in a very low concentration, but in the contaminated soils it occurs in locally elevated concentrations. The aim of this study was to examine the effect of nickel in the concentrations of 300 (very high, close to LOEC for reproduction) and 900 (extremely high, close to LOEC for mortality) mg/kg dry soil on the life history and acetylcholinesterase, catalase, and glutathione S-transferase activities in earthwormEisenia fetidaand to establish how nickel modifies the sensitivity to organophosphorous pesticide—diazinon. Cocoons production and juveniles’ number were significantly lower only in groups exposed to Ni in the concentration of 900 mg/kg dry soil for two months. Diazinon administration diminished the AChE activity in the GI tract and in the body wall. The interaction between diazinon and nickel was observed, and, in consequence, the AChE activity after the pesticide treatment was similar to controls in worms preexposed to nickel. Both pesticide administration and exposure to nickel caused an increase in the GST activity in examined organs and CAT activity in body wall. Both biometric and development data and simple enzymatic analysis, especially the AChE and GST, show a Ni pretreatment effect on the subsequent susceptibility to pesticide.
机译：镍在典型土壤中的含量非常低，但在受污染的土壤中镍的含量却在局部升高。这项研究的目的是研究300 mg / kg干燥土壤中的镍（非常高，接近LOEC的繁殖水平）和900（极高，接近LOEC的死亡率）对生活史和乙酰胆碱酯酶的影响EEisenia fetida中的磷酸，过氧化氢酶和谷胱甘肽S-转移酶活性，并确定镍如何改变对有机磷农药-二嗪农的敏感性。仅在暴露于镍的浓度为900 mg / kg干燥土壤两个月的组中，茧的产生和幼鱼的数量显着降低。二嗪农的施用减少了胃肠道和体壁中的AChE活性。观察到二嗪农与镍之间的相互作用，因此，农药处理后的AChE活性类似于预先暴露于镍的蠕虫中的对照。农药的施用和镍的暴露都导致被检查器官的GST活性和体壁CAT活性的增加。生物特征数据和发育数据以及简单的酶促分析（尤其是AChE和GST）都显示出Ni预处理对随后的农药敏感性的影响。

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