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首页> 外文期刊>Archives of Biological Sciences >USEFULNESS OF THE MYCOBACTERIUM TUBERCULOSIS DIRECT ASSAY IN EARLY DIAGNOSIS OF EXTRAPULMONARY TB
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USEFULNESS OF THE MYCOBACTERIUM TUBERCULOSIS DIRECT ASSAY IN EARLY DIAGNOSIS OF EXTRAPULMONARY TB

机译：结核分枝杆菌直接检查在肺外结核早期诊断中的实用性

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The aim of this study was to evaluate the in situ detection of living mycobacterium TB rRNA by the mycobacterium TB direct assay (MTD) and its clinical significance in the early diagnosis of extrapulmonary TB. Eighty-six patients were recruited from the Shanghai Public Health Clinical Center from June to November in 2010, having been diagnosed with extrapulmonary TB, including tuberculous peritonitis (n=22), lymphatic TB (n=21), tuberculous meningitis (n=15), HIV-associated TB (n=13), nephroTB (n=9), spinal TB (n=2), cutaneous TB (n=13), parotid TB (n=1), chest wall TB (n=1), intestinal TB (n=1). One hundred and five extrapulmonary specimens, including CSF, puncture fluid, drainage, pleural fluid, urine, secretion, ascites, lymphatic tissue and marrow were collected from the patients. The samples were examined using acid-fast stain, solid culture, liquid culture and MTD in parallel. In MTD, the target segments of MTB rRNA in either cultures or clinical specimens were amplified prior to being qualitatively detected with the hybridization protection assay (HPA). The sensitivities of MTD and acid-fast staining in liquid and solid cultures were 48.6%, 41.9%, 20.0% and 14.3%, respectively. MTD sensitivity was higher than that of the others and its specificity was 100%. We concluded that MTD rRNA detection is an effective, rapid, convenient, sensitive and reliable method for the early diagnosis of extrapulmonary TB.
机译：这项研究的目的是评估结核分枝杆菌直接测定法（MTD）现场检测活结核分枝杆菌rRNA的方法及其在肺外结核早期诊断中的临床意义。 2010年6月至11月，从上海公共卫生临床中心招募的86例患者被诊断出患有肺外结核，包括结核性腹膜炎（n = 22），淋巴结核（n = 21），结核性脑膜炎（n = 15） ），HIV相关结核（n = 13），肾结核（n = 9），脊柱结核（n = 2），皮肤结核（n = 13），腮腺结核（n = 1），胸壁结核（n = 1） ），肠结核（n = 1）。从患者中收集了一百零五个肺外标本，包括脑脊液，穿刺液，引流，胸膜液，尿液，分泌物，腹水，淋巴组织和骨髓。平行使用耐酸染色，固体培养，液体培养和MTD检查样品。在MTD中，先扩增培养物或临床标本中MTB rRNA的靶片段，然后再用杂交保护分析（HPA）进行定性检测。在液体和固体培养物中，MTD和耐酸染色的敏感性分别为48.6％，41.9％，20.0％和14.3％。 MTD的敏感性高于其他MTD，其特异性为100％。我们得出结论，MTD rRNA检测是一种早期肺外结核诊断的有效，快速，方便，灵敏和可靠的方法。

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