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首页> 外文期刊>Archives of Biological Sciences >THE CYTOTOXIC ACTIVITY OF TWO D-GALACTOSE-BINDING LECTINS PURIFIED FROM MARINE INVERTEBRATES
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THE CYTOTOXIC ACTIVITY OF TWO D-GALACTOSE-BINDING LECTINS PURIFIED FROM MARINE INVERTEBRATES

机译：海洋无脊椎动物纯化的两种D-半乳糖结合的凝集素的细胞毒活性

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The present investigation was undertaken in order to evaluate the cytotoxic effect of two D-galactose-binding lectins using the brine shrimp lethality bioassay technique. Both lectins were purified from the marine invertebrates, sea hare Aplysia kurodai eggs and polychaete Perineries nuntia by conventional affinity chromatography methods. The molecular mass of Aplysia kurodai egg lectin (AKL) was determined to be 32 kDa and 56 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis under reducing and non-reducing conditions, respectively. On the other hand, polychaete Perineries nuntia lectin (PnL) was determined to be 32 kDa in both reducing and non-reducing conditions. AKL and PnL showed strong agglutination activity against trypsinized and glutaraldehyde-fixed human and rabbit erythrocytes. AKL significantly affects the mortality rate of brine shrimp. Experimental results revealed that AKL was found to be more toxic (63.33% mortality) than PnL (33.33% mortality) and the mortality rate of brine shrimp nauplii was increased with the increase in concentration of lectins. These cytotoxic results indicate that future findings of lectin applications obtained from marine invertebrates may be of importance to clinical microbiology, and that they could have application as potent chemotherapeutic agents.
机译：进行本研究是为了使用盐水虾致死性生物测定技术评估两种D-半乳糖结合凝集素的细胞毒性作用。两种凝集素均通过常规亲和色谱法从海洋无脊椎动物，海兔黑棘海兔卵和多毛Per会阴提纯中纯化。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳分别在还原和非还原条件下，将黑海葵卵凝集素（AKL）的分子量分别确定为32 kDa和56 kDa。另一方面，在还原和非还原条件下，多毛Per会阴凝集素（PnL）被确定为32 kDa。 AKL和PnL对胰蛋白酶和戊二醛固定的人和兔红细胞显示强凝集活性。 AKL显着影响盐水虾的死亡率。实验结果表明，AKL的毒性（63.33％的死亡率）比PnL（33.33％的死亡率）更具毒性，盐水虾无节幼体的死亡率随着凝集素浓度的增加而增加。这些细胞毒性结果表明，从海洋无脊椎动物获得的凝集素应用的未来发现可能对临床微生物学很重要，并且它们可以作为有效的化学治疗剂应用。

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