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首页> 外文期刊>Archives of Applied Science Research >Statistical optimization of the production of a cellulase-free, thermoalkali- stable, salt- and solvent-tolerant xylanase from Bacillus halodurans by solid state fermentation
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Statistical optimization of the production of a cellulase-free, thermoalkali- stable, salt- and solvent-tolerant xylanase from Bacillus halodurans by solid state fermentation

机译：通过固态发酵从嗜碱性芽孢杆菌生产无纤维素酶，热碱稳定，耐盐和耐溶剂的木聚糖酶的统计优化

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A cellulase-free, thermo-alkali-stable, salt- and solvent-tolerant xylanase (Bhxyl) was produced from mutant strain of a newly isolated Bacillus halodurans FNP 135. Bhxyl showed optimal activity at 75 0C and was stable at pH 6-10, displaying 60% activity even at pH 12. Its T½ was 45 min at 70 0C. Bhxyl retained 76% activity in 1M NaCl and was highly tolerant ( 85%) to organic solvents (50% v/v) and surfactants (1%). Thin layer chromatography analysis revealed that xylose was the sole end product of xylan hydrolysis by the crude enzyme. Bhxyl yield was enhanced by mutation and optimization of process parameters for wheat bran based solid state fermentation (SSF) using Plackett-Burman design and response surface methodology. Under optimal conditions viz. incubation time, 68.40 h; sodium carbonate, 2.31% (w/w) and wheat bran: moisture ratio, 1:0.8 (w/v) in tap water, a yield of 5056 ± 96 U/g wheat bran, a 16 fold increase over unoptimized conditions, was obtained. Cost effective enhanced production of a highly stable xylanase was achieved for its biotechnological applications in pulp and paper industry and biomass degradation.
机译：从新分离的嗜盐芽孢杆菌FNP 135的突变菌株生产无纤维素酶，热碱稳定，耐盐和耐溶剂的木聚糖酶（Bhxyl）。Bhxyl在75 0C时显示最佳活性，在pH 6-10时稳定，即使在pH 12时也显示60％的活性。其T½在70 0C下为45分钟。 Bhxyl在1M NaCl中保留了76％的活性，对有机溶剂（50％v / v）和表面活性剂（1％）具有高度的耐受性（> 85％）。薄层色谱分析表明，木糖是木聚糖被粗酶水解的唯一最终产物。通过使用Plackett-Burman设计和响应面方法对基于麦麸的固态发酵（SSF）进行突变和优化工艺参数，提高了Bxxyl的产量。在最佳条件下。孵育时间68.40小时;碳酸钠，2.31％（w / w）和麦麸：水分比，自来水中的1：0.8（w / v），产量为5056±96 U / g麦麸，比未优化的条件提高了16倍获得。由于其在制浆造纸工业中的生物技术应用和生物质降解，实现了高成本效益的高稳定性木聚糖酶生产。

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