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首页> 外文期刊>Journal of industrial microbiology & biotechnology >Production and optimization of cellulase-free, alkali-stable xylanase by Bacillus pumilus SV-85S in submerged fermentation
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Production and optimization of cellulase-free, alkali-stable xylanase by Bacillus pumilus SV-85S in submerged fermentation

机译：短小芽孢杆菌SV-85S在深层发酵中生产和优化无纤维素酶，碱稳定的木聚糖酶

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摘要

This paper reports the production of a cellulase-free and alkali-stable xylanase in high titre from a newly isolated Bacillus pumilus SV-85S using cheap and easily available agro-residue wheat bran. Optimization of fermentation conditions enhanced the enzyme production to 2995.20 +/- A 200.00 IU/ml, which was 9.91-fold higher than the activity under unoptimized basal medium (302.2 IU/ml). Statistical optimization using response-surface methodology was employed to obtain a cumulative effect of peptone, yeast extract, and potassium nitrate (KNO3) on enzyme production. A 2(3) central composite design best optimized the nitrogen source at the 0 level for peptone and yeast extract and at the -alpha level for KNO3, along with 5.38-fold increase in xylanase activity. Addition of 0.1% tween 80 to the medium increased production by 1.5-fold. Optimum pH for xylanase was 6.0. The enzyme was 100% stable over the pH range from 5 to 11 for 1 h at 37A degrees C and it lost no activity, even after 3 h of incubation at pH 7, 8, and 9. Optimum temperature for the enzyme was 50A degrees C, but the enzyme displayed 78% residual activity even at 65A degrees C. The enzyme retained 50% activity after an incubation of 1 h at 60A degrees C. Characteristics of B. pumilus SV-85S xylanase, including its cellulase-free nature, stability in alkali over a long duration, along with high-level production, are particularly suited to the paper and pulp industry.
机译：本文报道了使用廉价且易于获得的农业残留小麦麸皮从新分离的短小芽孢杆菌SV-85S高滴度生产无纤维素酶和碱稳定的木聚糖酶的方法。发酵条件的优化提高了酶的产量至2995.20 +/- A 200.00 IU / ml，比未优化的基础培养基（302.2 IU / ml）下的酶活性高9.91倍。使用响应表面方法进行统计优化，以获取蛋白ept，酵母提取物和硝酸钾（KNO3）对酶产生的累积影响。 2（3）中央复合材料设计可将蛋白source和酵母提取物的0氮源和KNO3的-alpha氮源水平最佳化，同时木聚糖酶活性可提高5.38倍。在培养基中添加0.1％吐温80，产量提高了1.5倍。木聚糖酶的最佳pH为6.0。在37A的温度下5至11的pH范围内1小时，该酶100％稳定，即使在pH 7、8和9孵育3小时后，酶也没有失去活性。最适温度为50A。 C，但该酶即使在65A摄氏度下仍显示78％的残留活性。该酶在60A摄氏度下孵育1小时后仍保留50％的活性。短小芽孢杆菌SV-85S木聚糖酶的特性，包括其无纤维素酶的特性，长期稳定的碱稳定性以及高水平生产，特别适合造纸和纸浆工业。

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