...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Artificial cells, nanomedicine, and biotechnology. >3-Dimensional nano-fibre scaffold for ex vivo expansion of cord blood haematopoietic stem cells
【24h】

3-Dimensional nano-fibre scaffold for ex vivo expansion of cord blood haematopoietic stem cells

机译：用于脐血造血干细胞离体扩增的三维纳米纤维支架

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract Cord blood (CB) haematopoietic stem cell (HSC) is an alternative source of HSC transplantation. The limited cell number greatly restricts their clinic-scale therapeutic applications. The objective of this study was an ex vivo expansion of CB HSCs in a new three-dimensional polycaprolactone nano-scaffold coated with fibronectin (FN). First, we isolated CB CD34+ cells and cultured 10 days in presence of growth factors. The evaluation was performed by qRT-PCR, flow cytometry and clonogenicity. 3D PCL nano-scaffold coated with FN produced significantly higher total nucleated cells and CD34+ cells (p?p?CXCR-4, VLA-4, VLA-5 and LFA-1, and also HOXB-4, HOXA-9, BMI-1 and hTERT genes was higher in 3D than 2D. The CD13, CD14, CD33, CD34 and CD45 markers were significantly higher and CD2, CD3 and CD19 markers were significantly lower in 3D scaffold than 2D cell culture (p?p??.05). 3D PCL nano-scaffold coated with FN could better keep specifications homing and self renewality of CB HSCs after expansion.
机译：摘要脐血（CB）造血干细胞（HSC）是HSC移植的另一种来源。有限的细胞数量极大地限制了其临床规模的治疗应用。这项研究的目的是在一个新的三维三维纤维己内酯纳米骨架涂上纤连蛋白（FN）的体外CB HSCs扩展。首先，我们分离出CB CD34 +细胞，并在生长因子存在下培养10天。通过qRT-PCR，流式细胞仪和克隆形成性进行评估。涂有FN的3D PCL纳米支架产生的总有核细胞和CD34 +细胞明显更高（p？p？CXCR-4，VLA-4，VLA-5和LFA-1，以及HOXB-4，HOXA-9，BMI- 3D支架中的1和hTERT基因在3D中高于2D。在3D支架中，CD13，CD14，CD33，CD34和CD45标记显着较高，而CD2，CD3和CD19标记显着低于2D细胞培养（p？p？>？）。 05）。FN涂层的3D PCL纳米支架可以更好地保持CB HSC膨胀后的规格归位和自我更新。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号