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首页> 外文期刊>Asian journal of animal and veterinary advances >Isolation and Characterization of Marine Beneficial Bacteria from Petroleum Contaminated Sites for Better Environment of Animal Life
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Isolation and Characterization of Marine Beneficial Bacteria from Petroleum Contaminated Sites for Better Environment of Animal Life

机译：从石油污染场所分离和鉴定海洋有益细菌，以改善动物生活环境

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Polycyclic Aromatic Hydrocarbons (PAHs) are persistent hydrophobic organic pollutants ubiquitously found in the environment. PAHs consist of cytotoxic, mutagenic and carcinogenic properties and causes serious hazard to human health and environment. Hence, in this investigation eight phenanthrene (250 mg L-1) degrading bacterial strains were isolated by enrichment method from petroleum contaminated sludge samples. Among these isolates, one efficient degrading strain was identified and characterized by using morphological, biochemical, chemotaxonomic (FAMEs analysis) and molecular (16S rDNA sequencing) methods. Based on these studies the strain was identified as gram positive, motile, spore forming, pink pigmented cocci with oxidase negative reaction and utilized sugars such as arabinose, xylose and rhamnose as carbon source . It is susceptible to kanamycin, nalidixic acid and novobiocin type of antibiotics. The dominant fatty acid s found in this strain are 15:0 anteiso (44.16%), 15:0 iso (26.36%). Phylogenetic analysis of this strain showed 99% sequence similarity with Kocuria rosea and the strain name as PDM-7 was adopted. The degradation study illustrates that this strain utilizing about 82% of phenanthrene in six days. Therefore, this strain can be efficiently used for the bioremediation of PAHs contaminated marine environments and soils.
机译：多环芳烃（PAHs）是在环境中普遍发现的持久性疏水有机污染物。多环芳烃具有细胞毒性，致突变性和致癌性，会对人体健康和环境造成严重危害。因此，在这项研究中，通过富集方法从石油污染的污泥样品中分离了八种菲（250 mg L -1 ）降解细菌菌株。在这些分离物中，通过形态学，生化，化学分类学（FAMEs分析）和分子（16S rDNA测序）方法鉴定并鉴定了一种有效的降解菌株。基于这些研究，该菌株被鉴定为革兰氏阳性，活动性，孢子形成，带有氧化酶阴性反应的粉红色色素球菌，并利用诸如阿拉伯糖，木糖和鼠李糖的糖作为碳源。它对卡那霉素，萘啶酸和新霉素类抗生素敏感。该菌株中发现的主要脂肪酸为15：0异isoiso（44.16％），15：0 isoiso（26.36％）。该菌株的系统进化分析显示，与玫瑰红（Kocuria rosea）具有99％的序列相似性，并采用名称为PDM-7的菌株。降解研究表明，该菌株在六天内利用了约82％的菲。因此，该菌株可以有效地用于污染海洋环境和土壤的多环芳烃的生物修复。

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