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首页> 外文期刊>Asian journal of animal and veterinary advances >Development of a Gold Nanoparticle Based Lateral Flow Assay for Rapid Diagnosis of Contagious Agalactia in Goats
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Development of a Gold Nanoparticle Based Lateral Flow Assay for Rapid Diagnosis of Contagious Agalactia in Goats

机译：基于金纳米颗粒的侧向流动分析法快速诊断山羊接触性无乳症的开发

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摘要

A gold nanoparticle based lateral flow assay was developed for rapid serodiagnosis of contagious agalactia, an economically important mycoplasmal disease of small ruminants. Sonicated antigen of Mycoplasma agalactiae was used as the test reagent that was immobilized on nitrocellulose membrane along with the control line of goat IgG. The detection reagent, gold nanoparticle conjugated with anti-goat antibody was dried on the conjugate pad. During the assay, specific antibodies against M. agalactiae in the test serum that combined with the detection reagent were captured in the test line and detected visually by the development of a red line on nitrocellulose membrane. The gold conjugate captured in control line produced a red line regardless of the presence of specific antibodies that served as a procedural control. Serum samples collected from an experimentally infected goat were tested with the lateral flow assay and antibodies were detected from 9th day of infection and the assay was also evaluated using 100 goat sera samples. This is the first report regarding development of a gold nanoparticle based lateral flow assay for rapid diagnosis of contagious agalactia in goats. This study suggests that current lateral flow assay can be used as a user friendly diagnostic in laboratories lacking specialized equipments as well as for point of care diagnosis of contagious agalactia.
机译：开发了一种基于金纳米颗粒的侧向流动测定法，用于快速血清学诊断传染性无乳杆菌，这是一种经济上重要的小反刍动物支原体疾病。将无乳支原体的超声处理抗原与山羊IgG的对照品系一起固定在硝酸纤维素膜上作为测试试剂。将与抗山羊抗体缀合的检测试剂金纳米颗粒在缀合物垫上干燥。在测定过程中，将与检测试剂结合的测试血清中抗无乳分枝杆菌的特异性抗体捕获在测试线中，并通过硝化纤维素膜上的红线的显影目视检测。不管是否存在作为程序对照的特异性抗体，在对照线中捕获的金结合物都会产生红线。从实验感染的山羊收集的血清样品用侧流分析法进行测试，从感染的第9天开始检测抗体，并且还使用100个山羊血清样品评估该分析法。这是有关开发基于金纳米颗粒的侧向流动分析法以快速诊断山羊接触性无乳症的第一份报告。这项研究表明，当前的侧向血流分析可以在缺乏专用设备的实验室中用作用户友好的诊断方法，并且可以用于传染性无乳症的即时诊断。

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