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首页> 外文期刊>Asian journal of animal and veterinary advances >A Rapid Way to Map Outheld-Wing Mutation in Drosophila melanogaster
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A Rapid Way to Map Outheld-Wing Mutation in Drosophila melanogaster

机译：一种快速的方法绘制黑腹果蝇的悬突翼突变

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Map-based positional cloning of Drosophila melanogaster genes is hampered by both the time-consuming, labor-intensive, error-prone nature of traditional methods for genetic mapping-SNP mapping is costly and P element mapping is low-resolution for limit number of P elements. Here we used two strategies to locate a spontaneous mutant screened in our lab. One is P element mapping to map the mutant in a 33 k-interval on the right arm of the second chromosome; another is combination of P element mapping and a Single-Nucleotide Polymorphism (SNP) mapping together to map the mutant in a very small interval, about 5 kb, on the right arm of the second chromosome efficiently. At last, we guessed that the mutant phenotype maybe resulted from the overexpression of twist gene. Comparing the two strategies, the method combining the P insertion mapping and SNP mapping could ward off their drawbacks. Therefore, the strategy of combination of the two methods provides a rapid protocol for mapping mutations.
机译：传统的遗传作图方法耗时，费力，容易出错，这限制了果蝇基因的基于图的位置克隆-SNP作图成本高且P元素作图分辨率低，限制了P的数量元素。在这里，我们使用了两种策略来定位在我们的实验室中筛选的自发突变体。一种是P元件作图法，以33k间隔将突变体作图到第二条染色体的右臂上。另一个是将P元素定位和单核苷酸多态性（SNP）定位结合在一起，以很小的间隔（约5 kb）有效地将突变体定位在第二条染色体的右臂上。最后，我们推测突变表型可能是由于Twist基因的过表达所致。比较这两种策略，将P插入映射和SNP映射相结合的方法可以避免它们的缺点。因此，两种方法结合的策略为映射突变提供了快速的方案。

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