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An amperometric biosensor for choline determination prepared from choline oxidase immobilized in polypyrrole-polyvinylsulfonate film

机译:由固定在聚吡咯-聚乙烯磺酸盐薄膜中的胆碱氧化酶制备的用于测定胆碱的安培生物传感器

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In this paper, a novel amperometric choline biosensor with immobilization of choline oxidase on electrochemically polymerized polypyrrole–polyvinylsulphonate (PPy–PVS) film has been accomplished via the entrapment technique. The effects of pH and temperature were investigated and optimum parameters were found to be 9.0 and 60 °C, respectively. There are two linear parts in the region between 1.0 × 10 ?7 ? 1.0 × 10 ?6M (R2 = 0.997) and 1.0 × 10 ?5 ? 1.0 × 10 ?3 M (R2 = 0.986). The storage stability and operation stability of the enzyme electrode were also studied.
机译:在本文中,通过夹带技术完成了一种新型的安培胆碱生物传感器,其胆碱氧化酶固定在电化学聚合的聚吡咯-聚乙烯磺酸酯(PPy-PVS)膜上。研究了pH和温度的影响,发现最佳参数分别为9.0和60°C。在1.0×10?7?之间的区域中有两个线性部分。 1.0×10×6M(R2 = 0.997)和1.0×10×5Ω 1.0×10 3 M(R 2 = 0.986)。还研究了酶电极的储存稳定性和操作稳定性。

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