Biochemical Properties of Free and Immobilized Candida rugosa Lipase onto Alsub2/subOsub3/sub: A Comparative Study

Ye?im Ye?ilo?lu; Latif?ah ?it

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Biochemical Properties of Free and Immobilized Candida rugosa Lipase onto Alsub2/subOsub3/sub: A Comparative Study

机译：Al _{2 O _{3 上游离的固定化皱纹假丝酵母脂肪酶的生化特性：比较研究}}

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: Lipase from Candida rugosa (CRL) was immobilized by physical adsorption onto Al2O3. The immobilization yield was more than 95% during 30 min. The properties of free and immobilized enzyme were also searched and compared. The optimum pH was shifted from 7 to 8.5 by immobilization of the enzyme. The maximum activity of the free and the immobilized enzymes occured at 35 and 40°C, respectively.

机译：：通过物理吸附将皱纹假丝酵母（CRL）的脂肪酶固定在Al 2 O 3上。 30分钟内固定化率超过95％。还搜索并比较了游离和固定化酶的性质。通过固定化酶，最佳pH从7变为8.5。游离酶和固定化酶的最大活性分别出现在35和40°C下。

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《Artificial cells, blood substitutes, and immobilization biotechnology.》 |2011年第4期|共5页
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Ye?im Ye?ilo?lu; Latif?ah ?it;
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中图分类生物医学工程;
关键词
Al2O3Candida rugosa lipaseimmobilizationadsorptionstabilityoptimization;

机译：Al2O3皱纹假单胞菌脂肪酶的吸附稳定性优化;

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1. Biochemical properties of free and immobilized candida rugosa lipase onto Al 2O 3: A comparative study [J] . Ye?ilo?luY., ?itL. Artificial cells, blood substitutes, and immobilization biotechnology . 2011,第4期

机译：游离和固定化念珠菌脂肪酶在Al 2O 3上的生化特性：比较研究
2. Biochemical properties of free and immobilized Candida rugosa lipase onto Al2O3: a comparative study. [J] . Yesiloglu Y, Sit L Artificial cells, blood substitutes, and biotechnology . 2011,第4期

机译：游离和固定化念珠菌脂肪酶在Al2O3上的生化特性：一项比较研究。
3. Immobilization Studies and Biochemical Properties of Free and Immobilized Candida Rugosa Lipase onto Hydrophobic Group Carrying Polymeric Support [J] . Aysegul U. Metin Macromolecular Research . 2013,第2期

机译：游离和固定念珠菌脂肪酶在疏水性基团上的固定化研究及生化特性
4. Kinetic Studies of Lipase from Candida rugosa A Comparative Study Between Free and Immobilized Enzyme onto Porous Chitosan Beads [C] . GISELLA M. ZANIN, FLAVIO F. DE MORAES, HEIZIR F. DE CASTRO, Symposium on biotechnology for fuels and chemicals . 2001

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机译：使用不同方法固定在聚硅氧烷 - 聚乙烯醇混合载体上的假丝酵母脂肪酶性能的比较固定在聚硅氧烷上的假丝酵母脂肪酶的比较性能

Biochemical Properties of Free and Immobilized Candida rugosa Lipase onto Alsub2/subOsub3/sub: A Comparative Study

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