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首页> 外文期刊>Asian journal of andrology >Evaluation of the expression of sperm proteins in normozoospermic and asthenozoospermic men using monoclonal antibodies
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Evaluation of the expression of sperm proteins in normozoospermic and asthenozoospermic men using monoclonal antibodies

机译：使用单克隆抗体评估正常精子症和弱精子症男人的精子蛋白表达

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Recent studies have shown that infertility affects estimated 15% of all couples. Male infertility is the primary or contributory cause in 60% of these cases. Consequently, the application of assisted reproduction is increasing. These methods could benefit from an extended evaluation of sperm quality. For this reason, we analyzed sperm proteins from 30 men with normal spermiograms and 30 men with asthenozoospermia. Ejaculates of both groups were tested by flow cytometry (FCM) and fluorescence with a set of well-characterized anti-human sperm Hs-monoclonal antibodies (MoAbs), which were generated in our laboratory. No statistically significant differences were found between normospermics and asthenospermics in the expression of the sperm surface protein clusterin, evaluated with Hs-3 MoAb, and semenogelin, evaluated with Hs-9 MoAb. However, FCM revealed quantitative differences in the acrosomal proteins between normozoospermic and asthenozoospermic men, namely, in glyceraldehyde-3-phosphate dehydrogenase, evaluated with Hs-8 MoAb, valosin-containing protein, evaluated with Hs-14 MoAb, and ATP synthase (cAMP-dependent protein kinase II, PRKAR2A), evaluated with MoAb Hs-36. Asthenozoospermic men displayed a highly reduced expression of intra-acrosomal proteins, with a likely decrease in sperm quality, and thus a negative impact on successful reproduction. Asthenozoospermia seems to be a complex disorder involving intra-acrosomal proteins.
机译：最近的研究表明，不育症影响所有夫妇的15％。男性不育是其中60％的主要原因。因此，辅助再现的应用正在增加。这些方法可以受益于对精子质量的扩展评估。因此，我们分析了30名精子检查正常的男性和30名弱精子症的男性的精子蛋白。两组的射精均通过流式细胞仪（FCM）和荧光进行检测，并使用了一组在我们实验室中产生的，特征明确的抗人精子Hs单克隆抗体（MoAbs）。用Hs-3 MoAb评估精子表面蛋白簇蛋白和用Hs-9 MoAb评估精液蛋白的正常精子体和弱精子体之间在统计学上无显着差异。然而，FCM揭示了正常精子症和弱精子症人之间顶体蛋白质的定量差异，即使用Hs-8 MoAb评估的甘油醛-3-磷酸脱氢酶，使用Hs-14 MoAb评估的含缬氨酸的蛋白质和ATP合酶（cAMP）依赖蛋白激酶II（PRKAR2A），用MoAb Hs-36评估。弱精子症男性的精子内蛋白表达高度降低，精子质量可能下降，因此对成功繁殖产生负面影响。弱精子症似乎是一种复杂的疾病，涉及头内蛋白。

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