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首页> 外文期刊>Australian Journal of Crop Science >Determination of oligosaccharide fraction in a worldwide germplasm collection of chickpea (Cicer arietinum L.) using high performance liquid chromatography
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Determination of oligosaccharide fraction in a worldwide germplasm collection of chickpea (Cicer arietinum L.) using high performance liquid chromatography

机译:高效液相色谱法测定鹰嘴豆(Cicer arietinum L.)的全球种质资源集合中的寡糖组分

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Awareness on nutritive value and health benefits of legumes is of vital importance in order to increase the consumption of legumes in daily diet of the human beings. Despite of high nutritional value, it contains one of the most important antinutritional compounds such as the Raffinose Family Oligosaccharides (RFOs). The RFOs are predominantly found in the seeds of leguminous crops and are indigestible by humans and animals causing flatulence or diarrhea. In the present study, a total of 213 chickpea accessions were analysed for seed oligosaccharides (Sucrose, Raffinose, Ciceritol, and Stachyose) through high performance liquid chromatography with the aim of selecting promising accessions with low RFOs and high sucrose. Data with three replications was used to measure Pearson’s simple correlation co-efficient in order to establish the relationship among various oligosaccharide fractions. The results showed that the sucrose content ranged from 3.57 mg g-1 to 54.12 mg g-1 with 17 accessions contained more than 35 mg g-1 and 10 accessions had less than 10 mg g-1. The stachyose content ranged from 2.77 mg g-1 to 59.43 mg g-1 with 12 accessions had less than 11 mg g-1. Ciceritol was the main sugar found in all the samples ranged from 4.36 mg g-1 to 90.65 mg g-1. The identified germplasm accessions with unique sugar profiles especially those with low RFO will be valuable in breeding specialty chickpea for improved sugar content.
机译:对豆类的营养价值和健康益处的认识对于增加人类日常饮食中豆类的消费至关重要。尽管具有很高的营养价值,但它仍包含最重要的抗营养化合物之一,例如棉子糖家族寡糖(RFO)。 RFO主要存在于豆类作物的种子中,不被人类和动物消化,引起肠胃胀气或腹泻。在本研究中,通过高效液相色谱法分析了总共213个鹰嘴豆种质的种子寡糖(蔗糖,棉子糖,松香醇和水苏糖),目的是选择低RFO和高蔗糖的有前景种质。为了确定各种寡糖组分之间的关​​系,使用了三个重复的数据来测量Pearson的简单相关系数。结果表明,蔗糖含量在3.57 mg g-1至54.12 mg g-1之间,其中17种成分的含量超过35 mg g-1,而10种成分的含量低于10 mg g-1。水苏糖含量在2.77 mg g-1至59.43 mg g-1之间,有12种加入量少于11 mg g-1。柠檬醇是所有样品中发现的主要糖,含量范围为4.36 mg g-1至90.65 mg g-1。鉴定出的具有独特糖谱的种质材料,特别是那些具有低RFO的种质,对于提高糖含量的特种鹰嘴豆的育种具有重要价值。

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