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首页> 外文期刊>Australian Journal of Crop Science >Analysis of nuclear DNA content and chromosome number for screening genotypes and crosses in Annual Ryegrass (Lolium multiflorum Lam.)
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Analysis of nuclear DNA content and chromosome number for screening genotypes and crosses in Annual Ryegrass (Lolium multiflorum Lam.)

机译：分析一年生黑麦草（Lolium multiflorum Lam。）基因型和杂交的核DNA含量和染色体数

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摘要

Lolium multiflorum Lam. (annual ryegrass) is a forage grows in temperate regions of the world. In South America, annual forms of L. multiflorum exist and have been used as forage for a long time in Argentina, Chile, Uruguay, and Brazil. This study determined the nuclear DNA content and chromosome number for screening genotypes and crosses in L. multiflorum from Brazil. Analyses to estimate nuclear DNA content by flow cytometry were performed in young leaves of four parental genotypes and eight offspring resulting from their crosses. To the chromosome counting, the slides were prepared by flame drying technique and Giemsa staining. The genotypes were classified in the following categories: diploid, triploid, and tetraploid with 14, 21 and 28 chromosomes, respectively. The average content of DNA in diploids ranged from 5.42pg to 5.85pg. A single triploid plant showed 8.30pg. In tetraploids, the average DNA content ranged from 10.21pg to 10.95pg. Thus, the combination of analyses by DNA quantification and chromosome counting prove to be efficient for screening and diagnosing ploidy level of parents and crosses in Lolium. This information may be used reliably for prior selection of genotypes for the breeding program.
机译：黑麦草林。 （一年生黑麦草）是一种牧草，生长在世界温带地区。在南美洲，存在一年生的何首乌（L. multiflorum），并在阿根廷，智利，乌拉圭和巴西长期用作饲料。这项研究确定了用于筛选来自巴西的何首乌的基因型和杂交的核DNA含量和染色体数目。在四个亲本基因型和八个由其杂交产生的后代的幼叶中，进行了通过流式细胞术估计核DNA含量的分析。对于染色体计数，通过火焰干燥技术和Giemsa染色制备载玻片。基因型分为以下几类：二倍体，三倍体和四倍体，分别具有14、21和28条染色体。二倍体中DNA的平均含量为5.42pg至5.85pg。一棵三倍体植物显示8.30pg。在四倍体中，平均DNA含量在10.21pg至10.95pg之间。因此，通过DNA定量和染色体计数的分析相结合被证明对于筛选和诊断黑麦草亲本和杂交的倍性水平是有效的。该信息可以可靠地用于育种程序的基因型的事先选择。

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