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首页> 外文期刊>Australian Journal of Crop Science >Genetic diversity and relationships in wild species of Brassica and allied genera as revealed by cross-transferable genomic STMS marker assays
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Genetic diversity and relationships in wild species of Brassica and allied genera as revealed by cross-transferable genomic STMS marker assays

机译:交叉转移基因组STMS标记测定揭示了芸苔属及其相关属野生物种的遗传多样性和相关性

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One hundred and sixty one genomic STMS markers were tested from four different Brassica species (Brassica napus L., Brassica nigra (L.) Koch, Brassica rapa L. and Brassica oleracea L.) for cross-transferability to eleven different species of Brassica and allied genera. In total, 70 (43.5%) STMS markers showed cross-transferability to at least one of the eleven species which were considered for the study and it revealed a significant effect of taxonomic group on the proportion of markers amplified. Out of the 70 cross-transferable STMS markers, 32 (45.7%) markers were polymorphic and distinguished the studied taxa. The ‘B’ genome derived STMS markers showed lower level of cross-transferability as compared to those derived from ‘A’ and ‘C’ genomes of Brassica species. The cross-transferable STMS markers were applied to characterize the genetic variability and to evaluate the genetic relationships among the species using un- weighted pair group method average (UPGMA) analysis. The results indicated a high level of congruence with earlier reports and are in agreement with their recognized taxonomy.
机译:测试了来自四种不同芸苔属植物(甘蓝型油菜,芸苔属黑种(L.)科赫,芸苔属油菜和甘蓝型油菜)的161个基因组STMS标记物与11种不同芸苔属植物的交叉转移性。联盟属。总共有70个(43.5%)STMS标记与研究中考虑的11种物种中的至少一种表现出交叉转移性,并且揭示了分类组对扩增的标记比例的显着影响。在70个可交叉转移的STMS标记中,有32个(45.7%)标记是多态的,可以区分研究的类群。与源自芸苔属物种的“ A”和“ C”基因组的基因相比,来自“ B”基因组的STMS标记具有较低的交叉转移能力。使用非加权对分组平均法(UPGMA)分析,将可交叉转移的STMS标记用于表征遗传变异性并评估物种之间的遗传关系。结果表明与以前的报告高度一致,并且与它们公认的分类法一致。

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