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首页> 外文会议>2010 4th International Conference on Bioinformatics and Biomedical Engineering >Genetic Diversity and Relationships of Three Razor Clam Species (Sinonovacula constricta, Solen strictus and Solen grandis) Revealed by RAPD and ISSR Markers
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Genetic Diversity and Relationships of Three Razor Clam Species (Sinonovacula constricta, Solen strictus and Solen grandis) Revealed by RAPD and ISSR Markers

机译：RAPD和ISSR标记揭示的三种剃刀蛤类物种的遗传多样性和亲缘关系（Sinonovacula constricta，Solenstrictus和Solen grandis）

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Random amplified polymorphic DNA (RAPD) and Inter-simple sequence repeats (ISSR) markers were used to study the genetic diversity and relationships in three razor clam species (sinonovacula constricta, solen strictus and solen grandis). 14 random primers for RAPD analyzing generated 113 bands, out of which 105 (92.92%) were polymorphic loci and with an average of 8.1 bands per primer. Nine primers for ISSR analyzing generated 171 bands, out of which 169 (98.83%) were polymorphic loci and with an average of 19 bands per primer. Both Shannon's gene diversity index and Nei's gene diversity index within population comprehensively indicated that S. constricta possessed the lowest diversity, intermediate in Solen grandis and the highest in Solen strictus. The genetic distances among the three razor clam species calculated by RAPD and ISSR ranged from 0.4122 to 0.5505, and 0.2987 to 0.3842, respectively. Two UPGMA dendrograms of RAPD and ISSR were performed based on the Nei's genetic distances. Each dendrogram had two main clusters, one cluster including Solen strictus and Solen grandis, and the other was S. constricta. All of the results obtained in this study indicate that RAPD and ISSR markers are useful for genetic diversity analysis in razor clams.
机译：随机扩增的多晶型DNA（RAPD）和简单间序列重复（ISSR）标记用于研究三种剃刀蛤蜊物种（Sinonovacula Crantricta，Solen Strictus和Solen Grandis）的遗传多样性和关系。 14用于RAPD分析的随机引物产生113条带，其中105（92.92％）是多态基因座，平均每种引物为8.1条带。为ISSR分析产生的九个引物产生的171条带，其中169（98.83％）是多态基因座，平均每种引物为19条带。 Shannon的基因多样性指数和Nei的基因多样性指数全面表明S. Constricta拥有最低的多样性，苏珊祖母中级，最高的血统术。 RAPD和ISSR计算的三种剃刀蛤蜊物种中的遗传距离分别为0.4122至0.5505，分别为0.2987至0.3842。基于Nei的遗传距离来进行RAPD和ISSR的两个UPGMA星形图。每个树枝图都有两个主要集群，一个集群，包括血统标记和苏珊祖母，另一个是S. Constricta。本研究中获得的所有结果表明，RAZD和ISSR标记可用于剃刀蛤的遗传多样性分析。

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