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首页> 外文期刊>Biochemical Genetics >Genetic Diversity and Phylogenetic Relationships of Two Closely Related Northeast China Vicia Species Revealed with RAPD and ISSR Markers
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Genetic Diversity and Phylogenetic Relationships of Two Closely Related Northeast China Vicia Species Revealed with RAPD and ISSR Markers

机译：RAPD和ISSR标记揭示的东北两种密切亲缘蚕豆的遗传多样性和亲缘关系。

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摘要

RAPD and ISSR analyses revealed genetic diversity and relationships among 11 populations of two closely related northeast China Vicia species, Vicia ramuliflora and V. unijuga. Both methods yielded similar and complementary results, showing high genetic diversity. Vicia ramuliflora had 100% polymorphic loci in both RAPD and ISSR, and V. unijuga had 100% polymorphic loci for RAPD and 98.96% for ISSR. Genetic differentiation was moderate among populations of each species. Genetic variation was distributed mainly within populations for the two species. The high level of gene flow was important for the allocation of genetic variation. The UPGMA dendrogram and principal coordinates analysis at the level of individuals and populations showed that V. ramuliflora and V. unijuga were more closely related than either of them was to the outgroup species, V. cracca. The small molecular variance of V. ramuliflora and V. unijuga supports the conclusion that these two species had a common ancestor.
机译：RAPD和ISSR分析揭示了两个紧密相关的东北东北蚕豆物种Vicia ramuliflora和V. unijuga的11个种群之间的遗传多样性和相关性。两种方法均产生相似和互补的结果，显示出很高的遗传多样性。豌豆野豌豆在RAPD和ISSR中均具有100％的多态性位点，而单株弧菌对于RAPD具有100％的多态性位点，对于ISSR具有98.96％的多态性位点。每个物种的种群之间的遗传分化是中等的。遗传变异主要分布在两个物种的种群内。高水平的基因流动对于遗传变异的分配很重要。 UPGMA树状图和主坐标分析在个体和种群的水平上显示，拉莫利弗拉花和V. unijuga与外来物种V. cracca的相关性比它们中的任何一个都更紧密。雷公藤和单叶朱砂小分子差异支持了这两个物种具有共同祖先的结论。

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