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Genetic diversity and phylogenetic relationships of two closely related northeast China Vicia species revealed with RAPD and ISSR markers.

机译:遗传多样性和系统发育关系与RAPD和ISSR标记揭示了中国近地区蚕麦物种。

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摘要

RAPD and ISSR analyses revealed genetic diversity and relationships among 11 populations of two closely related northeast China Vicia species, Vicia ramuliflora and V. unijuga. Both methods yielded similar and complementary results, showing high genetic diversity. Vicia ramuliflora had 100% polymorphic loci in both RAPD and ISSR, and V. unijuga had 100% polymorphic loci for RAPD and 98.96% for ISSR. Genetic differentiation was moderate among populations of each species. Genetic variation was distributed mainly within populations for the two species. The high level of gene flow was important for the allocation of genetic variation. The UPGMA dendrogram and principal coordinates analysis at the level of individuals and populations showed that V. ramuliflora and V. unijuga were more closely related than either of them was to the outgroup species, V. cracca. The small molecular variance of V. ramuliflora and V. unijuga supports the conclusion that these two species had a common ancestor.
机译:RAPD和ISSR分析揭示了11个群体的遗传多样性和关系,其中两个密切相关的东北地区vicia种类,vicia ramuliflora和unijuga。 两种方法产生相似且互补的结果,显示出高遗传多样性。 Vicia Ramuliflora在RAPD和ISSR中有100%多态性基因座,V. Unijuga有100%的RAPD多态性基因座,ISSR为98.96%。 遗传分化在每种物种的群体中是中等的。 遗传变异主要分布在两种物种的群体中。 高水平的基因流对遗传变异的分配是重要的。 个人和群体水平的Upgma树木和主坐标分析显示,V.Mamuliflora和V. Unijuga比其中任何一个都与之对小组种类,V.Croacca。 V.Mamuliflora和V. Unijuga的小分子方差支持这两个物种具有共同的祖先的结论。

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  • 来源
    《Biochemical Genetics》 |2010年第6期|共17页
  • 作者

    Han Y; Wang HY;

  • 作者单位

    Southwest University of Science and Technology Mianyang Sichuan China.;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
  • 关键词

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