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首页> 外文期刊>Australian Journal of Crop Science >In vitro cultivation of Mouriri elliptica (Mart.) a species with alimentary and medicinal potential using alternative to agar media
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In vitro cultivation of Mouriri elliptica (Mart.) a species with alimentary and medicinal potential using alternative to agar media

机译：使用琼脂培养基的替代培养具有食用和药用潜力的Mouriri elliptica（Mart。）

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Alternative supports can be successfully used in place of agar for in vitro culture to increase seedling vigor. The objective of thepresent study was to evaluate the efficiency of alternative support materials compared to agar in the in vitro cultivation of Mouririelliptica (Mart.) in the absence or presence of naphthalene acetic acid (NAA). The alternative support materials used were medium-grain vermiculite, sugarcane (Saccharum spp. L.) bagasse and queen palm fiber [Syagrus romanzoffiana (Chamisso) Glassman]. Forthis, young plants with at least two axillary buds under in vivo conditions were transferred to in vitro condition considering fourreplications with three young plants. After 45 days was evaluated the growth and anatomical explant characteristics. Autoclavingthese alternative supports without the media and proceeding the explant desinfestation was an efficient method to contaminationcontrol and plant growth in a in vitro condition. No differences were observed between agar, vermiculite and sugarcane bagassecultures for most growth characteristics evaluated. Greater numbers of adventitious and secondary roots and greater root lengthwere significantly observed in plantlets grown in the presence of vermiculite and the absence of naphthalene acetic acid. In theagar culture, roots had weak points and poorly differentiated tissues, with parenchymal tissue predominating. The addition of 2.0mg L -1 naphthalene acetic acid used did not stimulate rooting in this specie. So, other auxin source and concentrations should beevaluated in future studies. Both support materials used, vermiculite and sugarcane bagasse, representing promising agarsubstitutes to obtain seedlings with roots.
机译：替代性支持物可以成功地代替琼脂用于体外培养，以增加幼苗活力。本研究的目的是评估在不存在或存在萘乙酸（NAA）的情况下在Mouririelliptica（Mart。）的体外培养中，与琼脂相比其他支持材料的效率。使用的替代载体材料是中粒ver石，甘蔗（Saccharum spp。L.）蔗渣和皇后棕榈纤维[Syagrus romanzoffiana（Chamisso）Glassman]。为此，考虑到与三个幼株的四个重复，将在体内条件下具有至少两个腋芽的幼株转移到体外条件。 45天后评估生长和解剖外植体特征。在没有培养基的情况下对这些替代支持物进行高压灭菌，并进行外植体杀菌处理，是在体外条件下控制污染和植物生长的有效方法。琼脂，ver石和甘蔗渣培养物之间的大多数生长特性未见差异。在存在ver石和不存在萘乙酸的情况下，观察到大量不定根和次生根，以及更长的根长。在茶色培养物中，根部薄弱且组织分化差，实质组织占主导地位。使用的2.0mg L -1萘乙酸的添加不会刺激该物种的生根。因此，其他生长素的来源和浓度应在以后的研究中进行评估。所用的两种支持材料，ver石和甘蔗渣，代表有希望的琼脂替代品，以获得具有根的幼苗。

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