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首页> 外文期刊>Australian Journal of Agricultural Engineering >Somatic embryogenesis and plantlet regeneration from juvenile leaf explants of two cassava cultivars (TME 419 & TMS 98/0505)
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Somatic embryogenesis and plantlet regeneration from juvenile leaf explants of two cassava cultivars (TME 419 & TMS 98/0505)

机译：两个木薯品种的幼叶外植体的体细胞胚发生和植株再生（TME 419和TMS 98/0505）

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摘要

In vitro regenerated juvenile plantlets from two cassava cultivars (TME 419 and TMS 98/0505) leaf lobes were used to induce organized embryogenic structures (OES) on DKW2 50P medium for 3 weeks under dimmed light conditions at 26±20C. Maximum stable frequencies of OES for the two cultivars were obtained with 5mg/L picloram. Friable embryogenic calluses (FEC) were further produced on GD2 50P medium supplemented with 500 μM tyrosine. The percentage plantlets recovered on hormone free MS2 agar medium of the two cassava cultivars were 85 and 80% respectively with more than 90% post acclimatization survivals.
机译：来自两个木薯品种（TME 419和TMS 98/0505）叶的离体再生幼苗被用来在昏暗的光照条件下（26±20℃）在DKW2 50P培养基上诱导有组织的胚发生结构（OES）3周。用5mg / L的吡咯仑获得两个品种的OES的最大稳定频率。在补充有500μM酪氨酸的GD2 50P培养基上进一步产生易碎的胚性愈伤组织（FEC）。在两个木薯品种的无激素MS2琼脂培养基上回收的小植株百分比分别为85％和80％，驯化后存活率超过90％。

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