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首页> 外文期刊>Australian Journal of Crop Science >Genetic diversity analysis of soybean (Glycine max (L.) Merr.) genotypes making use of SSR markers
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Genetic diversity analysis of soybean (Glycine max (L.) Merr.) genotypes making use of SSR markers

机译：利用SSR标记分析大豆（Glycine max（L.）Merr。）基因型的遗传多样性

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In this study, we aimed to investigate the genetic diversity and polymorphism among 30 soybean genotypes maintained by the ARC using simple sequence repeat (SSR) markers. Soybean genotypes were characterized using 20 SSR primers. DNA was extracted using the standard cetyl trimethylammonium bromide method and amplified using PCR. Allele size was determined via comparison with a 100 base pair (bp) DNA ladder. Molecular data were analyzed, and a dendrogram and matrix were generated using GGT 2.0 software. A total of 216 alleles with an average of 10.8 alleles per locus were detected. The allele sizes ranged between 2 and 33 bp with an average of 18.7 bp. The polymorphic information content among genotypes varied from 0.85 (Satt001) to 0.75 (Satt43) with an average of 0.716, and heterozygosity ranged from 0.87 to 0.78 with an average of 0.7485. The most diverse genotypes were B 66 S 31, 69S 7, and R5-4-2 M, which indicated the efficiency of the SSR markers for the detection of genetic diversity. The results of the current study revealed the diversity among the soybean genotypes tested, which might aid breeders in the future in the selection of parents for breeding.
机译：在这项研究中，我们旨在调查使用简单重复序列（SSR）标记由ARC维持的30个大豆基因型之间的遗传多样性和多态性。使用20个SSR引物表征大豆基因型。使用标准的十六烷基三甲基溴化铵方法提取DNA，并使用PCR进行扩增。通过与100个碱基对（bp）的DNA阶梯比较来确定等位基因的大小。分析了分子数据，并使用GGT 2.0软件生成了树状图和矩阵。总共检测到216个等位基因，每个基因座平均10.8个等位基因。等位基因大小在2到33 bp之间，平均18.7 bp。基因型之间的多态信息含量从0.85（Satt001）到0.75（Satt43）不等，平均值为0.716，杂合度在0.87到0.78之间，平均值为0.7485。最多样化的基因型是B 66 S 31、69S 7和R5-4-2 M，这表明SSR标记可用于检测遗传多样性。当前研究的结果表明，测试的大豆基因型之间存在多样性，这可能在将来帮助育种者选择育种亲本。

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