• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>IBRO Reports >A glycine transporter 2-Cre knock-in mouse line for glycinergic neuron-specific gene manipulation
【24h】

A glycine transporter 2-Cre knock-in mouse line for glycinergic neuron-specific gene manipulation

机译：用于甘氨酸能神经元特异性基因操纵的甘氨酸转运蛋白2-Cre敲入小鼠系

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Glycine is an inhibitory neurotransmitter in the brainstem and spinal cord. Glycine transporter 2 (GLYT2) is responsible for the uptake of extracellular glycine. GLYT2 is specifically expressed in glycinergic neurons and thus has been used as a marker of glycinergic neurons. Here, we generated GLYT2 promotor-driven Cre recombinase (Cre)-expressing mice (GLYT2-Cre knock-in mice) to develop a tool for manipulating gene expression in glycinergic neurons. Cre activity was examined by crossing the GLYT2-Cre knock-in mice with a Cre reporter mouse line, R26R, which express β-galactosidase (β-gal) in a Cre-dependent manner. X-gal staining of GLYT2-Cre/R26R double transgenic mouse brains and spinal cords revealed that the Cre activity was primarily distributed in the brainstem, cerebellum, and spinal cord. These areas are rich in glycinergic neurons. Furthermore, we performed immunohistochemistry for β-gal combined with in situ hybridization for GLYT2 in the GLYT2-Cre/R26R double transgenic mouse brains to determine whether Cre activity is specifically localized to glycinergic neurons. The β-gal protein and GLYT2 mRNAs were colocalized in the cerebellar Golgi cells, dorsal cochlear nucleus, gigantocellular reticular nucleus, spinal trigeminal nucleus, nucleus of the trapezoid body, and lateral lemniscus. More than 98% of the GLYT2 mRNA-expressing cells in these brain regions also expressed β-gal, whereas 90–98% of the β-gal-positive cells expressed the GLYT2 mRNAs. Thus, Cre activity is specifically localized to glycinergic neurons with high fidelity in the GLYT2-Cre knock-in mice. The GLYT2-Cre knock-in mouse line will be a useful tool for studying glycinergic neurons and neurotransmission.
机译：甘氨酸是脑干和脊髓中的抑制性神经递质。甘氨酸转运蛋白2（GLYT2）负责细胞外甘氨酸的摄取。 GLYT2在甘氨酸能神经元中特异性表达，因此已被用作甘氨酸能神经元的标记。在这里，我们生成了GLYT2启动子驱动的Cre重组酶（Cre）表达小鼠（GLYT2-Cre敲入小鼠），以开发用于操纵甘氨酸能神经元中基因表达的工具。通过将GLYT2-Cre敲入小鼠与以Cre依赖性方式表达β-半乳糖苷酶（β-gal）的Cre报告基因小鼠系R26R杂交，来检查Cre的活性。 GLYT2-Cre / R26R双转基因小鼠的大脑和脊髓的X-gal染色显示，Cre活性主要分布在脑干，小脑和脊髓中。这些区域富含甘氨酸能神经元。此外，我们对β-gal进行了免疫组织化学，并在GLYT2-Cre / R26R双转基因小鼠脑中对GLYT2进行了原位杂交，以确定Cre活性是否专门定位于甘氨酸能神经元。 β-gal蛋白和GLYT2 mRNA在小脑高尔基细胞，背侧耳蜗核，巨细胞网状核，脊柱三叉神经核，梯形体核和侧列环中共定位。在这些大脑区域中，超过98％的GLYT2 mRNA表达细胞也表达β-gal，而90-98％的β-gal阳性细胞表达GLYT2 mRNA。因此，在GLYT2-Cre敲入小鼠中，Cre活性以高保真度专门定位于甘氨酸能神经元。 GLYT2-Cre敲入小鼠系将是研究甘氨酸能神经元和神经传递的有用工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号