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首页> 外文期刊>IBRO Reports >MALDI mass spectrometry imaging of neuroprotective proteins against amyloid beta at a single cell resolution based on micro-scale metal pattern arrays
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MALDI mass spectrometry imaging of neuroprotective proteins against amyloid beta at a single cell resolution based on micro-scale metal pattern arrays

机译：基于微尺度金属图案阵列的单细胞分辨率下针对淀粉样蛋白β的神经保护蛋白的MALDI质谱成像

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Generation of neuronal cell type-specific BAC transgenic mouse models for brain mapping The mammalian central nervous system (CNS) contains an enor- mous variety of cell types, each with a complex connectivity. To understand the molecular mechanisms that contribute to the for- mation and function of the brain, it is important to know the precise expression of specific genes, and it is required the ability to identify, visualize and genetically manipulate each of the major CNS cell types. Bacterial artificial chromosome (BAC) transgenic mouse technique can provide one of the useful methods to visu- alize individual cells and their connectivity of cells in the brain. Since genomic fragments of BACs are large and contain most regu- latory sequences of a gene, they are most useful for inducing stable and tissue-specific expression in BAC transgenic mice. The aim of our study is to map the expression of the most important region-, cell type-specific genes in the developing CNS using BAC transgenic mouse technique. Here we show the expressions of 3 representing genes in the brain by generating the BAC transgenic mice that are incorporated EGFP reporter gene. Fezf2 which encodes a zinc-finger transcription factor is highly enriched in the deep-layer neuronal cells and corticospinal (CS) tract. The GFP in the Fezf2-GFP BAC transgenic mice is expressed in the deep-layer of cortex and CS tract as well. Adenosine A2a receptor (Adora2a) is specifically expressed in the striatum. The Adora2a-GFP BAC transgenic mice also show the GFP expression in the striatum. Ak4 which encodes an adeny- late kinase 4 is expressed in the pyramidal cells in the cerebral cortex and CA3 of the hippocampus. The GFP in Ak4-GFP BAC trans- genic mice is expressed in deeper layer of cerebral cortex and CA3 pyramidal cells in the hippocampus. The visualizing cell morphol- ogy and the connection of cells by BAC transgenic technique will provide more insight into the understanding of brain function.
机译：用于脑图绘制的特定神经元细胞类型BAC转基因小鼠模型的生成哺乳动物中枢神经系统（CNS）包含多种细胞类型，每种类型具有复杂的连通性。要了解有助于大脑形成和功能的分子机制，重要的是要知道特定基因的精确表达，并且需要具有识别，可视化和遗传操纵每种主要CNS细胞类型的能力。 。细菌人工染色体（BAC）转基因小鼠技术可以提供一种使单个细胞及其在大脑中的细胞连通性可视化的有用方法之一。由于BAC的基因组片段很大并且包含基因的大多数调控序列，因此它们对于在BAC转基因小鼠中诱导稳定的组织特异性表达最有用。我们研究的目的是使用BAC转基因小鼠技术在发育中的CNS中绘制最重要的区域，细胞类型特异性基因的表达图。在这里，我们通过生成整合了EGFP报告基因的BAC转基因小鼠，显示了3种代表基因在大脑中的表达。编码锌指转录因子的Fezf2在深层神经元细胞和皮质脊髓（CS）道中高度富集。 Fezf2-GFP BAC转基因小鼠中的GFP也在皮层和CS道的深层表达。腺苷A2a受体（Adora2a）在纹状体中特异性表达。 Adora2a-GFP BAC转基因小鼠也显示纹状体中的GFP表达。编码腺苷酸激酶4的Ak4在大脑皮层的锥体细胞和海马CA3中表达。 Ak4-GFP BAC转基因小鼠中的GFP在海马区大脑皮层和CA3锥体细胞的更深层表达。通过BAC转基因技术可视化细胞形态学和细胞连接，将为了解脑功能提供更多见识。

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