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首页> 外文期刊>Annals of Saudi medicine. >Time-to-detection of bacteria and yeast with the BACTEC FX versus BacT/Alert Virtuo blood culture systems
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Time-to-detection of bacteria and yeast with the BACTEC FX versus BacT/Alert Virtuo blood culture systems

机译：BACTEC FX与BacT / Alert Virtuo血液培养系统的细菌和酵母检测时间

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BACKGROUND: Bloodstream infections are associated with high rates of morbidity and mortality. Rapid detection of bloodstream infections is important in achieving better patient outcomes. OBJECTIVE: Compare the time-to-detection (TTD) of the new BacT/Alert Virtuo and the BACTEC FX automated blood culture systems. DESIGN: Prospective simulated comparison of two instruments using seeded samples. SETTING: Medical microbiology laboratory. METHODS: Blood culture bottles were seeded in triplicate with each of the standard ATCC strains of aerobes, anaerobes and yeast. TTD was calculated as the length of time from the beginning of culture incubation to the detection of bacterial growth. MAIN OUTCOME MEASURES: TTD for the various tested organisms on the two microbial detection systems. RESULTS: The 99 bottles of seeded blood cultures incubated in each of the blood culture systems included 21 anaerobic, 39 aerobic and 39 pediatric bottles. The BacT/Alert Virtuo system exhibited significantly shorter TTD for 72.7 % of the tested organisms compared to BACTEC FX system with a median difference in mean TTD of 2.1 hours (interquartile range: 1.5-3.5 hours). The BACTEC FX system was faster in 15.2% (5/33) of microorganisms, with a median difference in mean TTD of 25.9 hours (IQR: 9.1-29.2 hours). CONCLUSION: TTD was significantly shorter for most of the microorganisms tested on the new BacT/Alert Virtuo system compared to the BACTEC FX system. LIMITATIONS: Use of simulated cultures to assess TTD may not precisely represent clinical blood cultures. CONFLICT OF INTEREST: None.
机译：背景：血流感染与高发病率和高死亡率相关。快速检测血流感染对改善患者预后至关重要。目的：比较新型BacT / Alert Virtuo和BACTEC FX自动血液培养系统的检测时间（TTD）。设计：使用种子样本对两种仪器进行前瞻性模拟比较。地点：医学微生物学实验室。方法：将血培养瓶分别与标准ATCC需氧菌，厌氧菌和酵母菌一式三份接种。 TTD计算为从培养开始到检测细菌生长所需的时间。主要观察指标：TTD用于两种微生物检测系统上各种被测生物。结果：在每个血液培养系统中培养的99瓶接种血培养物包括21厌氧，39有氧和39儿科瓶。与BACTEC FX系统相比，BacT / Alert Virtuo系统对72.7％的被测生物显示出显着更短的TTD，平均TTD的中位数差异为2.1小时（四分位数范围：1.5-3.5小时）。 BACTEC FX系统在15.2％（5/33）的微生物中速度更快，平均TTD的中位数差异为25.9小时（IQR：9.1-29.2小时）。结论：与BACTEC FX系统相比，在新的BacT / Alert Virtuo系统上测试的大多数微生物的TTD显着缩短。局限性：使用模拟培养物评估TTD可能无法准确代表临床血液培养物。利益冲突：无。

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