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首页> 外文期刊>Frontiers in Microbiology >In vitro Evaluation of BACT/ALERT? VIRTUO?, BACT/ALERT 3D?, and BACTEC? FX Automated Blood Culture Systems for Detection of Microbial Pathogens Using Simulated Human Blood Samples
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In vitro Evaluation of BACT/ALERT? VIRTUO?, BACT/ALERT 3D?, and BACTEC? FX Automated Blood Culture Systems for Detection of Microbial Pathogens Using Simulated Human Blood Samples

机译：体外对BACT / ALERT的评价？ VIRTUO？，BACT / ALERT 3D？和BACTEC？ FX自动化血液培养系统，用于使用模拟人血样本检测微生物病原体

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Blood culture (BC) is still the standard for diagnosing bloodstream infections (BSIs), especially those caused by bacteria and fungi. Infection-complicating sepsis or septic shock often occurs at BSI onset, making necessary to improve the diagnostic yield of positive BCs. Among the BC systems currently available, the BACT/ALERT? VIRTUO? (VIRTUO) system has been developed to shorten time to detection (TTD) of positive BCs. In this study, we assessed TTD for 330 clinically relevant species including 14 Gram-positive, 14 Gram-negative, and 5 yeast isolates in spiked human blood samples that were tested in parallel with VIRTUO BACT/ALERT? 3D (BTA3D) and BACTEC? FX (BACTEC) systems. We inoculated 30 colony-forming unit (CFU) from each microbial suspension into BACT/ALERT? Plus or BACTEC? Plus (aerobic/anaerobic or pediatric) BC bottles, and we used two different blood volumes to simulate, respectively, the BCs collected from adult and pediatric patients. Of 2,610 bottles tested, 2,600 (99.6%) signaled positive in the three systems. Only the BACTEC system did not detect Staphylococcus lugdunensis isolates in anaerobic bottles. Among adult simulated cultures, the median TTD was significantly shorter for aerobic/anaerobic bottles incubated in VIRTUO (11.6 h and 10.1 h) compared to bottles incubated in either BTA3D (13.3 and 12.3 h) or BACTEC (13.5 and 12.2 h) system. Among pediatric simulated cultures, the median TTD was significantly shorter for bottles incubated in VIRTUO (11.2 h) compared to bottles incubated in either the BTA3D (13.0 h) or BACTEC (12.5 h) system. Compared to BTA3D and/or BACTEC systems, VIRTUO allowed faster growth detection for most of the 33 microbial species tested. Notable examples were Salmonella spp. (7.4 h by VIRTUO vs. 10.1 h and 9.2 h by either BTA3D or BACTEC) and Streptococcus agalactiae (8.1 h by VIRTUO vs. 10.3 and 9.4 h by either BTA3D or BACTEC). The few notable exceptions included Stenotrophomonas maltophilia and some Candida species. Together, these findings confirm that VIRTUO has greater potential of improving the laboratory detection of bacteremia and fungemia than the progenitor BTA3D or the competitor BACTEC system.
机译：血培养（BC）仍然是诊断血流感染（BSI）的标准，尤其是细菌和真菌引起的那些。感染并发症败血症或败血性休克常在BSI发作时发生，因此有必要提高阳性BCs的诊断率。在当前可用的BC系统中，BACT / ALERT是？ VIRTUO？ （VIRTUO）系统已经开发出来，可以缩短阳性BC的检测时间（TTD）。在这项研究中，我们评估了加标人体血液样品中330种临床相关物种的TTD，包括14革兰氏阳性，14革兰氏阴性和5种酵母分离株，并与VIRTUO BACT / ALERT平行进行了测试。 3D（BTA3D）和BACTEC？ FX（BACTEC）系统。我们将每种微生物悬液中的30个菌落形成单位（CFU）接种到BACT / ALERT中？加号还是BACTEC？加上（有氧/厌氧或小儿）BC瓶，我们分别使用了两种不同的血量来模拟从成人和儿科患者身上收集的BC。在测试的2,610瓶中，有3,600瓶中有2,600瓶（99.6％）表示阳性。仅BACTEC系统未在厌氧瓶中检测到lugdunensis葡萄球菌分离株。在成人模拟培养物中，与在BTA3D（13.3和12.3 h）或BACTEC（13.5和12.2 h）系统中孵育的有氧/厌氧瓶相比，在VIRTUO（11.6 h和10.1 h）中孵育的中位TTD明显更短。在儿科模拟培养物中，与在BTA3D（13.0 h）或BACTEC（12.5 h）系统中孵育的瓶子相比，在VIRTUO中孵育的瓶子（11.2 h）的中位TTD明显短。与BTA3D和/或BACTEC系统相比，VIRTUO可以对测试的33种微生物中的大多数进行更快的生长检测。著名的例子是沙门氏菌。 （VIRTUO为7.4小时，而BTA3D或BACTEC为10.1小时和9.2小时）和无乳链球菌（VIRTUO为8.1小时，而BTA3D或BACTEC为10.3和9.4小时）。少数值得注意的例外包括嗜麦芽窄食单胞菌和某些假丝酵母菌。综合起来，这些发现证实，VIRTUO比BTA3D祖先或竞争对手BACTEC系统具有更大的潜力，可以改善实验室对菌血症和真菌病的检测。

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