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首页> 外文期刊>Indian Journal of Medical Microbiology >Diagnostic performance of mini parasep? solvent-free foecal parasite concentrator for the diagnosis of intestinal parasitic infections
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Diagnostic performance of mini parasep? solvent-free foecal parasite concentrator for the diagnosis of intestinal parasitic infections

机译:迷你性瘫痪的诊断性能?无溶剂粪便寄生虫浓缩仪用于诊断肠道寄生虫感染

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Introduction: Various stool concentration techniques have been used to increase the microscopic detection of parasites. We assessed the enclosed, single-vial, Mini Parasepsup?/sup technique in comparison to the currently used coprodiagnosis procedures. Materials and Methods: A total of 150 stool samples were collected. Samples concentrated by formol–ether method (FEM) and Mini Parasepsup?/sup SF foecal concentrator and unconcentrated samples were subjected to wet mount, iodine mount microscopy and smear examination by modified acid-fast staining. Results: Direct wet mount detected 72 positive samples (48.6%), whereas 77 (51.3%) and 80 (53.3%) samples were detected by FEM and Mini Parasepsup?/sup SF methods, respectively. The sensitivity of detection of parasites was 98.7%, 95% and 90.1% with Mini Parasepsup?/sup, FEM and direct microscopy, respectively. A clearer background with less foecal debris and a better yield of Hymenolepis nana, Trichuris trichiura, Entamoeba coli and Giardia lamblia were noted with Mini Parasepsup?/sup. Conclusion: Mini Parasepsup?/sup SF technique is simple, rapid and less cumbersome than conventional diagnostics, making it suitable for routine use. In addition, it offers higher sensitivity and better background clearance than both direct stool examination and FEM.
机译:简介:各种粪便浓缩技术已被用于增加对寄生虫的显微检测。与目前使用的辅助诊断方法相比,我们评估了封闭的单瓶Mini Parasep ?技术。材料和方法:总共收集了150个粪便样品。用福尔摩醚法(FEM)和Mini Parasep ? SF粪便浓缩仪浓缩的样品和未浓缩的样品经过湿法固定,碘固定显微镜和涂片检查,并经改良的耐酸染色。结果:直接湿法安装检测到72个阳性样品(48.6%),而通过FEM和Mini Parasep SF方法分别检测到77个(51.3%)和80个(53.3%)样品。 Mini Parasep ?,FEM和直接显微镜检测寄生虫的灵敏度分别为98.7%,95%和90.1%。使用Mini Parasep ?可以观察到更清晰的背景,且粪便碎片更少,且Hymenolepis nana,Trichuris trichiura,Entamoeba coli和Giardia lamblia的产量更高。结论:与常规诊断相比,Mini Parasep ? SF技术简单,快速且麻烦,因此适合常规使用。此外,与直接粪便检查和FEM相比,它具有更高的灵敏度和更好的背景清除率。

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