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首页> 外文期刊>Indonesian Journal of Forestry Research >EXPRESSION PATTERNS OF PHOSPHATE TRANSPORTER GENE ECGPT ASSOCIATED WITH THE SALT STRESS RESPONSE IN PERENNIAL EUCALYPT TREE HYBRID CLONES
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EXPRESSION PATTERNS OF PHOSPHATE TRANSPORTER GENE ECGPT ASSOCIATED WITH THE SALT STRESS RESPONSE IN PERENNIAL EUCALYPT TREE HYBRID CLONES

机译：盐胁迫对多年生桉树杂种无性系中磷酸转运蛋白基因ECGPT表达的影响。

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Salinity effects on plant can often be related to mineral ion content alteration, including phosphate. Under saline conditions, phosphate levels were reported to decrease in plants. Such effects could indirectly affect intracellular phosphate levels, leading to phosphate deficiency, which in turn leads to increased activities of phosphate uptake mechanisms. This research was aimed to investigate the effects of salinity on the expression changes of phosphate transporter genes isolated from E. camaldulensis x E. globulus hybrid clones subjected to salt concentrations of 0 (control), 50, 100, and 150 mMNaCl. Fragments of genes (1164bp long and encodes 304 amino acid polypeptides) known to be involved in phosphate uptake were identified and isolated by RACE from these hybrids, encoding a Phosphate Transporter ( Ecg PT). Expression studies using Northern-Blot analysis revealed that the expression of Ecg PT was found to be affected by salt, suggesting a direct effect of salinity on phosphate uptake. Ecg PT was expressed differently in different clones, indicating different degrees of phosphate transporter activation in order to tolerate salt stress. Molecular data are discussed in relation to measurements of ion levels in different organs of different clones and under various salt regimes during the course of the hydroponic experiment.
机译：盐分对植物的影响通常可能与矿物质离子含量的变化有关，包括磷酸盐。据报道，在盐水条件下，植物中的磷酸盐水平降低。这种作用可能间接影响细胞内磷酸盐的水平，导致磷酸盐缺乏，进而导致磷酸盐摄取机制的活性增加。这项研究旨在调查盐度对盐浓度分别为0（对照），50、100和150 mMNaCl的卡那杜氏x球藻杂种克隆的磷酸盐转运蛋白基因表达变化的影响。通过RACE从这些杂种中鉴定并分离了已知参与磷酸盐吸收的基因片段（长1164bp，编码304个氨基酸多肽），编码磷酸盐转运蛋白（Ecg PT）。使用Northern-Blot分析进行的表达研究表明，发现Ecg PT的表达受盐影响，这表明盐度对磷酸盐吸收具有直接影响。 Ecg PT在不同克隆中的表达不同，表明为了耐受盐胁迫，磷酸盐转运蛋白的活化程度不同。在水培试验过程中，讨论了与不同克隆的不同器官中以及各种盐制度下离子水平的测量有关的分子数据。

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