• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Indian Journal of Virology >Molecular Characterization of a Nucleopolyhedrovirus Newly Isolated from Ophiusa disjungens in China
【24h】

Molecular Characterization of a Nucleopolyhedrovirus Newly Isolated from Ophiusa disjungens in China

机译：新分离自中国蛇毛藻的一种核多角体病毒的分子特征

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Ophiusa disjungens nucleopolyhedrovirus (OpdiNPV) was newly found in Guangdong Province, China. Using BamHI, EcoRI, HindIII, PstI, XhoI, XbaI digestion, the size of the OpdiNPV genome was estimated to be 92,000 base pair. The pstI-G genomic fragment of OpdiNPV was cloned and sequenced. Searches of databases identified at least four open reading frames (ORFs) within this fragment. These ORFs encode odv-e66 (EU 623602), p87/vp80 (EU 732665), odv-ec43 ({"type":"entrez-nucleotide","attrs":{"text":"EU617337","term_id":"185179253","term_text":"EU617337"}}EU617337) and ac108 gene (EU 732666) respectively. The phylogenetic tree of NPVs based on the combined sequences of odv-ec43 and ac108 indicated that OpdiNPV was most closely related to Mamestra configurata NPV-A and Mamestra configurata NPV-B. The characterization of OpdiNPV in this paper would provide better understanding molecular properties of this virus and be helpful in the development of the newly isolated virus as a biopesticide or an engineered pesticide to control more species of insect pests.
机译：Ophiusa disjungens核多角体病毒（OpdiNPV）在中国广东省新发现。使用BamHI，EcoRI，HindIII，PstI，XhoI，XbaI消化，OpdiNPV基因组的大小估计为92,000个碱基对。克隆并测序了OpdiNPV的pstI-G基因组片段。对数据库的搜索确定了该片段内的至少四个开放阅读框（ORF）。这些ORF编码odv-e66（EU 623602），p87 / vp80（EU 732665），odv-ec43（{“ type”：“ entrez-nucleotide”，“ attrs”：{“ text”：“ EU617337”，“ term_id” ：“ 185179253”，“ term_text”：“ EU617337”}} EU617337）和ac108基因（EU 732666）。基于odv-ec43和ac108组合序列的NPV的系统发育树表明，OpdiNPV与Mamestra configurata NPV-A和Mamestra configurata NPV-B关系最密切。本文对OpdiNPV的表征将提供对这种病毒的分子特性的更好理解，并有助于开发新分离的病毒作为生物农药或工程农药来控制更多种类的害虫。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号