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首页> 外文期刊>Infection and Drug Resistance >Comparative analysis of the pathogenicity between multidrug-resistant Acinetobacter baumannii clinical isolates: isolation of highly pathogenic multidrug-resistant A. baumannii and experimental therapeutics with fourth-generation cephalosporin cefozopran
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Comparative analysis of the pathogenicity between multidrug-resistant Acinetobacter baumannii clinical isolates: isolation of highly pathogenic multidrug-resistant A. baumannii and experimental therapeutics with fourth-generation cephalosporin cefozopran

机译：多重耐药的鲍曼不动杆菌临床分离株之间的致病性比较分析：高致病性多重耐药的鲍曼不动杆菌的分离和第四代头孢菌素头孢佐普兰的实验治疗方法

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Introduction: The pathogenicity of fatal-outbreak Acinetobacter baumannii isolates has not been fully investigated. This study aimed to compare the pathogenicity between A. baumannii clinical isolates, including multidrug-resistant A. baumannii (MDRA). Materials and methods: Antibiotic susceptibility was determined by the broth microdilution method, and drug-resistant genes were characterized by PCR and sequencing. The pathogenicity of A. baumannii and antibiotic responses were evaluated using the Galleria mellonella infection model. Clinical isolates from an A. baumannii outbreak at our hospital were categorized using the pulse-field gel electrophoresis. Of the 16 isolated A. baumannii clones, 12 clones were resistant to carbapenems (meropenem and imipenem), of which 10 clones were also resistant to amikacin and ciprofloxacin (MDRAs). MDRAs had OXA-51-like β-lactamase gene harboring an insertion sequence in the promoter region and armA gene encoding 16S rRNA methyltransferase. Results: Carbapenem- and/or amikacin-resistant A. baumannii were more pathogenic than carbapenem- and/or amikacin-sensitive A. baumannii in G. mellonella . MDRA isolate TK1033 was more virulent than other A. baumannii isolates. However, TK1033 was sensitive to the fourth-generation cephalosporin cefozopran in addition to minocycline, tigecycline, and polymyxins (colistin and polymyxins B) in vitro and in vivo in the MDRA- G. mellonella infection model. Conclusion: Differences in pathogenicity among carbapenem-resistant A. baumannii clones are consistent with heterogeneous clinical outcomes. Strain TK1033, isolated frequently during the outbreak, was the most virulent, whereas preoutbreak isolate TK1032 was less virulent than other A. baumannii isolates. Infection by high-virulence isolates may be more prevalent during outbreaks. These strains may prove valuable for investigating MDRA virulence and novel therapeutics.
机译：简介：致命暴发鲍曼不动杆菌的致病性尚未得到充分研究。这项研究旨在比较鲍曼不动杆菌临床分离株（包括多重耐药鲍曼不动杆菌（MDRA））的致病性。材料和方法：用肉汤微量稀释法测定抗生素敏感性，并通过PCR和测序鉴定耐药基因。鲍曼不动杆菌的致病性和抗生素反应是通过梅勒埃勒菌感染模型评估的。使用脉冲场凝胶电泳对我院鲍曼不动杆菌暴发的临床分离株进行分类。在16个分离的鲍曼不动杆菌中，有12个克隆对碳青霉烯类（美罗培南和亚胺培南）有抗性，其中10个克隆也对阿米卡星和环丙沙星（MDRA）有抗性。 MDRA具有在启动子区域具有插入序列的OXA-51样β-内酰胺酶基因和编码16S rRNA甲基转移酶的armA基因。结果：耐碳青霉素和/或阿米卡星的鲍曼不动杆菌比致病菌碳青霉烯和/或阿米卡星的鲍曼不动杆菌对病原菌的致病性更高。 MDRA分离株TK1033比其他鲍曼不动杆菌分离株更具毒性。但是，在MDRA-M。mellonella感染模型中，除了米诺环素，替加环素和多粘菌素（colistin和多粘菌素B）外，TK1033还对第四代头孢菌素头孢唑仑敏感。结论：耐碳青霉烯的鲍曼不动杆菌克隆之间的致病性差异与异质临床结果一致。暴发期间经常分离出的菌株TK1033是最强毒的，而暴发前分离株TK1032的毒力低于其他鲍曼不动杆菌。在爆发期间，高毒力菌株的感染可能更为普遍。这些菌株可能证明对研究MDRA毒力和新型治疗方法有价值。

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