• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infectious Diseases and Therapy >Molecular Characterization of Pneumococcal Surface Protein A (PspA), Serotype Distribution and Antibiotic Susceptibility of Streptococcus pneumoniae Strains Isolated from Pakistan
【24h】

Molecular Characterization of Pneumococcal Surface Protein A (PspA), Serotype Distribution and Antibiotic Susceptibility of Streptococcus pneumoniae Strains Isolated from Pakistan

机译：巴基斯坦分离的肺炎链球菌肺炎球菌表面蛋白A（PspA），血清型分布和抗生素敏感性的分子表征

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

IntroductionPakistan has one of the highest burdens of pneumococcal diseases in the world, but unfortunately studies in this demanding research area are limited in the region. Pneumococcal surface protein A (PspA) is the next generation pneumococcal vaccine candidate as the protein locates on the Streptococcus pneumoniae surface. Its gene, pspA , might be encoded by all pneumococci, and the protein has proven immunogenicity. The molecular characterization of PspA, pneumococcal serotype distribution and antibiotic susceptibility are important for regional diversity studies. MethodsIn this study, we examined 38 pneumococcal isolates from pneumococcal diseased (pneumonia/meningitis) patients blood or cerebrospinal fluid. There were no specific inclusion or exclusion criteria, but all?the?individuals [ages 1?month to 12?years (male/female)] had undergone no antibiotic treatment in at least the past 3?months and had no vaccination history. We investigated the serotype distribution, antibiotic susceptibility, prevalence of the PspA family and its active domain's fusion, expression and antigenicity. ResultsOur finding shows that serotype 19F is the most prevalent (23.6%) followed by 18B (15.78%) (non-vaccine type) in all isolated pneumococcal strains. All strains were susceptible to chloramphenicol and linezolid, while 80% were resistant to gentamycin. Genotyping revealed that?~?80% ( N =?31/38) of pneumococcal strains produce PspA belonging to family 2 and clade 3. We further selected three active domains of PspA (family 2 and clade 3) by in silico analysis, merged together into a fusion gene for expression study, and its antigenicity was analyzed by Western blotting. ConclusionSerotypes 19F and 18B (non-vaccine type) are the most prevalent in the Pakistani pneumococcal isolates. The PspA family 2 proteins produced by Pakistani pneumococcal isolates have high sequence homologies with each other and differ from those produced by strains isolated in the rest of the world. The PspA fusion peptide had a proven antigenic response in western blotting, with no considerable correlation among pneumococcal serotypes, antibiotic susceptibility and PspA family/clade distribution.
机译：简介巴基斯坦是世界上肺炎球菌疾病负担最重的国家之一，但不幸的是，这一要求严格的研究领域的研究在该地区受到限制。肺炎球菌表面蛋白A（PspA）是下一代肺炎球菌疫苗候选者，因为该蛋白位于肺炎链球菌表面。它的基因pspA可能由所有肺炎球菌编码，并且该蛋白质已证明具有免疫原性。 PspA的分子特征，肺炎球菌血清型分布和抗生素敏感性对区域多样性研究很重要。方法在这项研究中，我们检查了38例来自肺炎球菌病（肺炎/脑膜炎）患者血液或脑脊液的肺炎球菌分离株。没有具体的纳入或排除标准，但是所有的个体[年龄在1个月至12岁之间（男性/女性）]至少在过去的3个月内没有接受过抗生素治疗，也没有疫苗接种史。我们调查了血清型分布，抗生素敏感性，PspA家族的患病率及其活性域的融合，表达和抗原性。结果我们的发现表明，在所有分离的肺炎球菌菌株中，血清型19F最普遍（23.6％），其次是18B（15.78％）（非疫苗型）。所有菌株均对氯霉素和利奈唑胺敏感，而80％对庆大霉素耐药。基因分型显示，约80％（N = 31/38）的肺炎球菌菌株产生PspA，属于家族2和进化枝3。我们通过计算机分析进一步选择了PspA的三个活性域（家族2和进化枝3），合并融合成融合基因用于表达研究，并通过蛋白质印迹分析其抗原性。结论19F和18B血清型（非疫苗型）在巴基斯坦肺炎球菌分离株中最普遍。巴基斯坦肺炎球菌分离株产生的PspA家族2蛋白彼此具有高度序列同源性，与世界其他地区分离出的菌株产生的同源性不同。 PspA融合肽在Western印迹中已证明具有抗原反应，在肺炎球菌血清型，抗生素敏感性和PspA家族/进化枝分布之间无显着相关性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号