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首页> 外文期刊>Infection and Drug Resistance >Comparative evaluation of methods for the detection of biofilm formation in coagulase-negative staphylococci and correlation with antibiogram
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Comparative evaluation of methods for the detection of biofilm formation in coagulase-negative staphylococci and correlation with antibiogram

机译：凝固酶阴性葡萄球菌生物膜形成检测方法的比较评价及与抗菌素的相关性

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Introduction: Coagulase-negative staphylococci (CNS) are normal commensals of human skin and mucous membranes. The objective of the study was to determine the prevalence of CNS among clinical isolates, characterize them up to species level, compare the three conventional methods for detection of biofilm formation, and study their antimicrobial susceptibility pattern. Materials and methods: CNS were obtained from various clinical samples including blood, urine, central venous catheter tips, endotracheal tube aspirate, and pus during a 1-year period (July 1, 2014, to June 30, 2015). Characterization up to species level was done using biochemical tests, and biofilm formation was detected by tube adherence, Congo red agar, and tissue culture plate method. Antimicrobial susceptibility testing was performed following Clinical and Laboratory Standards Institute guidelines. Results: A total of 71 CNS isolates, comprising of seven species were obtained. Staphylococcus epidermidis was the most common species followed by S. saprophyticus and S. haemolyticus . We detected biofilm formation in 71.8% of isolates. Considering the fact that tissue culture plate method is the gold standard, sensitivity of tube adherence method and Congo red agar method was found as 82% and 78%, respectively. The isolates exhibited high resistance toward penicillin (90%), azithromycin (60%), co-trimoxazole (60%), and ceftriaxone (40%), while all were susceptible to vancomycin and linezolid. Biofilm former isolates showed higher resistance than the non-formers. Conclusion: Among 71 CNS isolated, S. epidermidis was the most common isolate followed by S. saprophyticus and S. haemolyticus . Biofilm formation was detected in 71.8% of the isolates. All of the methods were effective in detecting biofilm-producing CNS strains. The antimicrobial resistance was significantly higher in biofilm formers than non-formers.
机译：简介：凝固酶阴性葡萄球菌（CNS）是人类皮肤和粘膜的正常代名词。这项研究的目的是确定临床分离株中CNS的流行程度，对它们进行物种鉴定，比较三种检测生物膜形成的常规方法，并研究其抗菌敏感性模式。材料和方法：在1年期间（2014年7月1日至2015年6月30日），从包括血液，尿液，中心静脉导管尖端，气管内导管抽吸物和脓液在内的各种临床样本中获得CNS。使用生化测试对物种进行了鉴定，并通过试管粘附，刚果红琼脂和组织培养板法检测了生物膜的形成。抗菌素敏感性测试是根据临床和实验室标准协会的指南进行的。结果：共获得71种CNS分离株，其中包括7种。表皮葡萄球菌是最常见的物种，其次是腐生链球菌和溶血链球菌。我们在71.8％的分离物中检测到生物膜形成。考虑到组织培养板法是金标准，管粘附法和刚果红琼脂法的灵敏度分别为82％和78％。分离株对青霉素（90％），阿奇霉素（60％），复方新诺明（60％）和头孢曲松（40％）表现出高抗性，而所有菌株均对万古霉素和利奈唑胺敏感。生物膜前分离物显示出比非前者更高的抗性。结论：在分离出的71种中枢神经系统中，表皮葡萄球菌是最常见的分离株，其次是腐生链球菌和溶血链球菌。在71.8％的分离物中检测到生物膜形成。所有这些方法都可有效检测产生生物膜的CNS菌株。生物膜形成剂中的抗药性显着高于非成膜剂。

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