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首页> 外文期刊>Integrative Medicine Research >Effect of Chinese herbal compound Tengmei decotion on IL-17/NF-κB signal pathway in synovium tissue of rat arthritis models induced by type {II} collage
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Effect of Chinese herbal compound Tengmei decotion on IL-17/NF-κB signal pathway in synovium tissue of rat arthritis models induced by type {II} collage

机译：中药藤美汤对Ⅱ型胶原致关节炎模型滑膜组织中IL-17 /NF-κB信号通路的影响

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Purpose: To investigate the biological effect of IL-17/NF- B immune inflammatory pathway on pathological damagein synovium tissue of Rheumatoid arthritis (RA) and study inregulation mechanism of Chinese herbal compound Tengmeidecotion for IL-17/NF- B signal pathway.Methods: To establish collagen II-induced rats arthritis(CIA)models. The successful models of SD rats wererandomly divided into model group, positive group, and high-,medium- groups of Chinese medicine, 6 rats in each group. Thenormal control and model groups were given distilled water(10ml kg-1 d) by gavage. The positive drug group was givenleflunomide (1.87g kg-1 d) by gavage. The high and mediumdose Chinese medicine groups were given crude medicine of31.8 g kg-1 d and 15.9 g kg-1 d by gavage. After twelve weeksof treatment intervention, all SD rats were executed, theblood and synovium tissue samples were kept for detectingIL-17, NF-KB P65 mRNA transcription and protein expressionby RT-PCR, WESTEN BLOT and ELISA Analysis, and detectinginflammatory infiltration in synovium tissue by Histopathologicalanalysis.Results: (1)Compared to the normal control group, levelsof mRNA transcription and protein expression of IL-17and NF- B P65 were significantly up-regulated (P < 0.01) in themodel group. Compared to the model group, levels of mRNAtranscription and protein expression of IL-17 and NF- B P65were significantly down-regulated in the positive and Chinesemedicine groups (P < 0.01).(2) Histopathological analysis displayedthat mild hyperplasia of epithelial cells covering thearticular cartilage synovium, joint cavity narrowing, and mildinflammatory lymphocytes infiltration in model group, withjoint lesions improved in treatment groups.Conclusion: The molecular mechanisms of Chinese herbalTengmei decotion in inhibiting immune inflammatory pathologicaldamage in synovium of CIA rats models related to itseffects on IL-17/NF- B pathway.
机译：目的：探讨IL-17 / NF-B免疫炎性通路对类风湿关节炎（RA）滑膜组织病理损伤的生物学作用，并研究中药复方腾美汤对IL-17 / NF-B信号通路的调节机制。 ：建立胶原Ⅱ诱导的大鼠关节炎（CIA）模型。成功的SD大鼠模型随机分为模型组，阳性组和中，高，中组，每组6只。正常对照组和模型组分别通过管饲法给予蒸馏水（10ml kg-1 d）。阳性药物组通过管饲法给予来氟米特（1.87g kg-1 d）。高剂量和中剂量中药组分别通过管饲法分别给予31.8 g kg-1 d和15.9 g kg-1 d的原料药。经过十二周的治疗干预后，处死所有SD大鼠，保留血液和滑膜组织样品，以通过RT-PCR，WESTEN BLOT和ELISA分析检测IL-17，NF-KB P65 mRNA的转录和蛋白质表达，并通过滑液检测滑膜组织中的炎症浸润组织病理学分析。结果：（1）模型组与正常对照组相比，IL-17和NF-B P65的mRNA转录水平和蛋白表达明显上调（P <0.01）。与模型组相比，阳性和中药组IL-17和NF-B P65的mRNA转录水平和蛋白表达水平明显下调（P <0.01）。（2）组织病理学分析显示，覆盖关节的上皮细胞轻度增生模型组软骨滑膜，关节腔变窄，轻度炎症淋巴细胞浸润，治疗组关节病变改善。 NF-B途径。

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