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首页> 外文期刊>International Journal of Biodiversity and Conservation >Genetic variation of barley germplasm from Turkey assessed by chloroplast microsatellite markers
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Genetic variation of barley germplasm from Turkey assessed by chloroplast microsatellite markers

机译：叶绿体微卫星标记评估土耳其大麦种质的遗传变异

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Five chloroplast DNA microsatellite loci were tested to analyze cytoplasmic variation in a set of 94 barley accessions; 23?Hordeum vulgare ssp. spontaneum?(wild) from Southeastern Anatolia, 47Hordeum vulgare ssp.vulgare?landraces and 24 Turkish barley cultivars. Fifteen (15) haplotypes were detected. Within wild barley, two of the five loci were polymorphic with two or three alleles and four haplotypes were found, giving a gene diversity value of 0.31. One of these haplotypes was found in 13 Turkish cultivars. Two loci were polymorphic in the landraces, giving three haplotypes and a gene diversity value of 0.33. One of haplotypes found in landraces was observed in a Turkish cultivar. All loci were polymorphic in 24 Turkish cultivars and the numbers of alleles per locus ranged from two to three alleles, with a gene diversity value of 0.61. Of the six haplotypes detected in Turkish cultivars, three were unique to three cultivars. The chloroplast microsatellite loci were found multiallelic and variable within?H. vulgare ssp. vulgare?and?H. vulgare ssp. spontaneum?intraspesific accessions and useful to compare degrees of genetic variation between accessions. The results improved the knowledge in genetic variation of CRIFC seed genebank’s barley accessions and could be informative for broadening the genetic base of barley.
机译：测试了五个叶绿体DNA微卫星基因座，以分析一组94个大麦种质中的细胞质变异。 23？大麦来自东南安纳托利亚的spontaneum？（野生），47个大麦属种，地方品种和24个土耳其大麦品种。检测到十五（15）个单倍型。在野生大麦中，五个基因座中的两个具有两个或三个等位基因多态性，并发现了四个单倍型，基因多样性值为0.31。在13个土耳其品种中发现了其中一种单倍型。两个基因座在地方品种中是多态的，给出了三个单倍型，基因多样性值为0.33。在土耳其品种中发现了在地方品种中发现的单倍型之一。所有位点在24个土耳其品种中都是多态的，每个位点的等位基因数目为2-3个等位基因，基因多样性值为0.61。在土耳其品种中检测到的六种单体型中，三种对三种品种而言是独特的。发现叶绿体微卫星基因座是多等位基因，在ΔH内变化。庸俗的庸俗的？庸俗的自发性内生种质，可用于比较种质间遗传变异的程度。结果提高了对CRIFC种子大麦种质遗传变异的认识，并可能为拓宽大麦的遗传基础提供信息。

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