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首页> 外文期刊>International Journal of Biotechnology and Molecular Biology Research >Sourcing starter cultures for Parkia biglobosa fermentation I: Phylogenic grouping of Bacillus species from commercial iru samples
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Sourcing starter cultures for Parkia biglobosa fermentation I: Phylogenic grouping of Bacillus species from commercial iru samples

机译：大叶Parkia发酵的发酵原料培养物I：商业iru样品中芽孢杆菌属的系统发生分组

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摘要

Twenty five isolates of Bacillus species were obtained from ten commercial samples of ‘iru’ (fermented African locust bean), after heat-treatment at 80°C for 30 min. All the isolates were Gram-positive, spore-former, catalase-positive, non-motile rods. The phylogenic relationship between the isolates was studied by repetitive PCR fingerprinting using the (GTG)5 primer, referred to as (GTG)5-PCR fingerprinting and 16S rRNA gene sequencing analyses. The (GTG)5-PCR fingerprinting resulted in grouping of the isolates into 12 phylogenetic groups. Based on the 16S rRNA gene sequence analysis, most of the Bacillus isolates were found to be closely related to Bacillus subtilis, while strain 8B was closely related to Bacillus licheniformis.
机译：在80°C的温度下热处理30分钟后，从十份商业化的“ iru”（非洲刺槐豆）样品中获得了25种芽孢杆菌分离株。所有分离物均为革兰氏阳性，孢子形成，过氧化氢酶阳性，无运动杆。通过使用（GTG）5引物的重复PCR指纹分析（称为（GTG）5-PCR指纹分析和16S rRNA基因测序分析）研究了分离物之间的系统关系。 （GTG）5-PCR指纹图谱可将分离株分为12个系统发育组。根据16S rRNA基因序列分析，发现大多数分离的芽孢杆菌与枯草芽孢杆菌密切相关，而菌株8B与地衣芽孢杆菌密切相关。

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