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首页> 外文期刊>International Journal of Biomedical Science >Developmental Changes of Catecholamine-mediating Enzyme - Dopamine-β- Hydroxylase and Its Cofactors in Central and Peripheral Tissues and Serum of Long-Evans Rats
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Developmental Changes of Catecholamine-mediating Enzyme - Dopamine-β- Hydroxylase and Its Cofactors in Central and Peripheral Tissues and Serum of Long-Evans Rats

机译：儿茶酚胺介导酶多巴胺-β-羟化酶及其辅因子在长埃文斯大鼠中，外周组织和血清中的发育变化

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Dopoamine-β-hydroxylase (DBH) is a catecholamine-synthesizing enzyme which catalyzes the formation of norepinephrine from dopamine. Fifty nine Long-Evans rats of 1 week-old were used to grow on normal diets in 7 different developmental stages, viz., 15 rats in 1 week-old group, 9 rats in 2 weeks-old group, and the remaining 35 rats were divided equally into five groups, 7 rats in each group of 5-, 8-, 12-, 15- and 27-weeks old for systematic developmental studies of DBH. At the end of each developmental period, weights of rats were recorded for that specific group and they were sacrificed. The brain tissues (caudate nucleus, hypothalamus, brain stem, colliculi, cerebral cortex and cerebellum) and peripheral tissues (liver, heart, kidney, adrenal, spleen, pancreas, lung and small intestine) and serum were collected. The brain tissues had the highest activity (expressed as nmole/min/g of wet weight tissue) of DBH at 5 weeks of age. The hypothalamus had the highest activities (11.5 ± 2.2) and the lowest activities were found in the cerebellum (5.7 ± 0.9). The peripheral tissues also showed the peak DBH activities at 5 weeks of age and adrenals had the highest activities (59.2 ± 7.0) among the central and peripheral tissues. The serum DBH activities were relatively low (1.3 ± 0.2) as compared to those in all other tissues. The highest serum DBH activities (1.28 ± 21 nmole/min/ml of serum) were also found in the 5- weeks-old rats. The specific activities of DBH were also measured in various developmental stages and the results were found to be in agreement with the DBH activities expressed in terms of gram of tissues or milliliter of serum. The Km and Vmax values for DBH were measured in the serum samples of each group and the highest Vmax values (78.3 ± 21.2 pmol/min/mg protein) were obtained at 5 weeks of age; while the lowest Km values (0.52 ± 0.04 mM) were obtained at this stage of age.
机译：多巴胺-β-羟化酶（DBH）是一种儿茶酚胺合成酶，可催化多巴胺形成去甲肾上腺素。 1周龄的59只Long-Evans大鼠在七个不同的发育阶段以正常饮食生长，即1周龄组15只大鼠，2周龄组9只大鼠，其余35只大鼠将其平均分为五组，每组分别为5、8、8、12、15和27周龄的7只大鼠，用于系统性DBH发育研究。在每个发育期结束时，记录该特定组大鼠的体重并处死。收集脑组织（尾状核，下丘脑，脑干，col，大脑皮层和小脑）和周围组织（肝，心脏，肾脏，肾上腺，脾脏，胰腺，肺和小肠）和血清。在5周龄时，脑组织具有最高的DBH活性（表示为nmole / min / g湿重组织）。下丘脑活动最高（11.5±2.2），而小脑活动最低（5.7±0.9）。周围组织在5周龄时也显示出峰值的DBH活性，而在中央和周围组织中，肾上腺的活性最高（59.2±7.0）。与所有其他组织相比，血清DBH活性相对较低（1.3±0.2）。在5周龄的大鼠中也发现最高的血清DBH活性（1.28±21nmol / min / ml血清）。在各个发育阶段还测量了DBH的比活性，发现结果与以组织克数或血清毫升数表示的DBH活性一致。测量每组血清样本中DBH的Km和Vmax值，并在5周龄时获得最高Vmax值（78.3±21.2 pmol / min / mg蛋白）;而在此年龄段，获得最低的Km值（0.52±0.04 mM）。

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