Microsatellites also known as simple sequence repeats (SSRs) are found in DNA sequences. These repeats consist of short motifs of 1-6 bp and play important role in population genetics, phylogenetics and also in the development of molecular markers. In this study chloroplastic SSRs (cpSSRs) in the chloroplast genome of Ptilidium pulcherrimum , downloaded from the National Center for Biotechnology Information (NCBI), were detected. The chloroplast genome sequence of P . pulcherrimum was mined with the help of a Perl script named MISA. A total of 23 perfect cpSSRs were detected in 119.007 kb sequence mined showing density of 1 SSR/5.17 kb. Depending on the repeat units, the length of SSRs found to be 12 bp for mono and tri, 12 to “22 bp for di, 12 to 16 bp for tetra nucleotide repeats. Penta and hexanucleotide repeats were completely absent in chloroplast genome of P . pulcherrimum . Dinucleotide repeats were the most frequent repeat type (47.83%) followed by tri (21.74%) and tetranucleotide (21.74%) repeats. Out of 23 SSRs detected, PCR primers were successfully designed for 22 (95.65%) cpSSRs. DOI: http://dx.doi.org/10.3126/ije.v3i3.11063 International Journal of Environment Vol.3(3) 2014: 50-58.
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机译：在DNA序列中发现了微卫星，也称为简单序列重复（SSR）。这些重复序列由1-6 bp的短基序组成，在种群遗传学，系统发育学以及分子标记的开发中起着重要作用。在这项研究中，检测到从美国国家生物技术信息中心（NCBI）下载的Ptilidium pulcherrimum的叶绿体基因组中的叶绿体SSR（cpSSRs）。拟南芥叶绿体基因组序列。在名为MISA的Perl脚本的帮助下开采了pulcherrimum。在119.007 kb的序列中共检测到23个完美的cpSSR，密度为1 SSR / 5.17 kb。取决于重复单元，发现SSR的长度对于单核苷酸和三核苷酸为12bp，对于二核苷酸重复为12至“ 22bp，对于二核苷酸重复为12至16bp。 P的叶绿体基因组中完全没有五核苷酸和六核苷酸重复。白头翁。二核苷酸重复是最常见的重复类型（47.83％），其次是三核苷酸（21.74％）和四核苷酸（21.74％）重复。在检测到的23个SSR中，成功设计了22个（95.65％）cpSSR的PCR引物。 DOI：http：//dx.doi.org/10.3126/ije.v3i3.11063 International Journal of Environment Vol.3（3）2014：50-58。
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