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首页> 外文期刊>International Journal of Electrochemical Science >Comparative Study on Voltammetric and Spectrofluorimetric Methods for Fluorescein Detection
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Comparative Study on Voltammetric and Spectrofluorimetric Methods for Fluorescein Detection

机译：伏安法和荧光光谱法检测荧光素的比较研究

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The aim of this research was assessment of suitability of both methods voltammetric andspectrofluorimetric to fluorescein detection. Electrochemical activity of the analyte was examined inthe supporting electrolytes of three different pH values due to a strong dependence of fluoresceinproperties on pH of the sample. Moreover, the electrochemical behavior of fluorescein was studiedusing different materials of working electrode – graphite paste, graphene paste and glassy carbon.Experiments performed using voltammetric methods indicated that fluorescein is electrochemicallyactive. The influence of pH on its electrochemical activity was confirmed. Fluorescein undergoes one-or two-step irreversible electrochemical oxidation in the considered potential range from 0 to +1.4 V,exhibiting the decrease of peak current with the increase of sample pH. Differential pulse voltammetricmeasurements enabled successful detection of fluorescein at micromolar concentration levels atgraphene and graphite sensors, with the following limit of detection values: for graphene sensors7.4 μM at pH 4.5, 34.55 μM at pH 7, 23.42 μM at pH 9; for graphite sensors 6.44 μM at pH 4.5,42.27 μM at pH 7, 95.12 μM at pH 9, whereas results obtained using glassy carbon electrodes wereless satisfactory. Comparative study of electrochemical and optical methods for fluorescein detectionindicated voltammetry as more suitable for measurements in the acidic solutions, whereasspectrofluorimetry was more advantageous in the case of alkaline media. Thus, we conclude that forquantitative detection of fluorescein in aqueous solutions, both voltammetric and spectrofluorimetricmethods are suitable, since complement each other – when associated, they allow to detect fluoresceinin wide pH range. On the basis of the obtained results we stated that pH value of the sample affects theutility of each method due to its effect on electrochemical and optical features of fluorescein.Therefore, when choosing the most adequate method – electrochemical or optical, for measurementsbased on fluorescein detection, pH of the sample should be taken into account, primarily.
机译：这项研究的目的是评估伏安法和荧光光谱法对荧光素检测的适用性。由于荧光素特性对样品pH的强烈依赖性，在三种不同pH值的支持电解质中检查了分析物的电化学活性。此外，使用不同的工作电极材料（石墨糊，石墨烯糊和玻璃碳）研究了荧光素的电化学行为。使用伏安法进行的实验表明，荧光素具有电化学活性。证实了pH对其电化学活性的影响。荧光素在0至+1.4 V的考虑电位范围内经历了一步或两步不可逆的电化学氧化，表现出随着样品pH值的增加峰值电流的减少。差分脉冲伏安法测量能够成功检测到微摩尔浓度水平的石墨烯和石墨传感器上的荧光素，检测值具有以下限制：对于石墨烯传感器，pH 4.5时为7.4μM，pH 7时为34.55μM，pH 9时为23.42μM;石墨传感器的pH值为6.44μM，pH为7时为4.5.42.27μM，pH为9时为95.12μM，而使用玻璃碳电极的结果却不尽人意。电化学和光学方法用于荧光素检测的比较研究表明伏安法更适合在酸性溶液中进行测量，而荧光光谱法在碱性介质中更有利。因此，我们得出结论，对水溶液中的荧光素进行定量检测，伏安法和荧光分光光度法都是合适的，因为它们可以相互补充–当相互关联时，它们可以在宽的pH范围内检测荧光素。根据获得的结果，我们指出样品的pH值因其对荧光素的电化学和光学特征的影响而影响每种方法的实用性。因此，在选择最合适的方法时，应采用电化学或光学方法进行基于荧光素检测的测量，首先应考虑样品的pH。

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