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首页> 外文期刊>International Journal of Electrochemical Science >Development of a Voltammetric Amine Oxidase-Modified Biosensor for the Determination of Biogenic Amines in Food
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Development of a Voltammetric Amine Oxidase-Modified Biosensor for the Determination of Biogenic Amines in Food

机译：伏安胺氧化酶修饰生物传感器的开发，用于食品中生物胺的测定

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A novel, stable biosensor for the detection of biogenic amines (BAs) was designed by immobilizingpea seedling amine oxidase (PSAO) on MnO2 modified carbon paste electrodes (CPEs). Hydrogenperoxide one of the products of the enzymatic reaction - was the electrochemically activeintermediate and was detected at + 400 mV vs. Ag/AgCl with differential pulse voltammetry (DPV). Immobilization of the enzyme was achieved with a Nafion membrane, which provides excellentbiocompatibility as well as good fixation properties. The biosensor was tested with different substrates,such as cadaverine, putrescine, tyramine, and histamine to obtain information about substratespecificity. A linear correlation was shown from 30 88 g/mL for cadaverine and 24 67 g/mL forputrescine. The limits of detection (LOD) were determined to be 10 g/mL for cadaverine and8 g/mL for putrescine and the limits of quantification (LOQ) were calculated to be 30 g/mL and24 g/mL respectively. Immobilized PSAO was inhibited by the irreversible non-competitive inhibitorsemicarbazide to prove that the observed signals were enzymatically generated. Common interferingagents such as uric acid, acetylsalicylic acid, and 4-acetamidophenol were checked on their influenceon the performance of the sensor. The sensor was used to quantify BAs in commercial fish sauce. Theaccuracy of the results was verified using the standard addition method.
机译：通过将豌豆幼苗胺氧化酶（PSAO）固定在MnO2修饰的碳糊电极（CPE）上，设计了一种新颖，稳定的生物胺（BAs）检测传感器。酶促反应的产物之一是过氧化氢，它是电化学活性中间体，用差分脉冲伏安法（DPV）在相对于Ag / AgCl的+ 400 mV处检测到。用Nafion膜固定了酶，该膜提供了出色的生物相容性和良好的固定特性。用不同的底物（例如尸胺，腐胺，酪胺和组胺）对生物传感器进行了测试，以获得有关底物特异性的信息。尸胺含量为30 88 g / mL，而腐胺含量为24 67 g / mL，线性相关。尸胺的检出限（LOD）确定为10 g / mL，腐胺的检出限（LOQ）计算为30 g / mL和24 g / mL。固定的PSAO被不可逆的非竞争性抑制剂氨基脲抑制，以证明观察到的信号是酶促产生的。检查了常见的干扰剂，例如尿酸，乙酰水杨酸和4-乙酰氨基苯酚，它们对传感器性能的影响。该传感器用于量化商业鱼露中的BA。使用标准添加方法验证了结果的准确性。

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