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A Novel Approach to Facile Synthesis and Biosensing of the Protein-Regulated Graphene

机译：蛋白质调控的石墨烯的简便合成和生物传感的新方法。

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In this study, we synthesized graphene through a green approach in which consuming of the chemical materials in production process decreased and reached to the lowest value. Bovine Serum Albumin (BSA) was used as the stabilizer for exfoliated graphene sheets. Synthesis of the functionalized graphene was confirmed by uv-vis spectroscopy. The physical and chemical properties of the synthesized graphene were compared with conventional graphene oxide. Atomic force microcopy (AFM) was utilized to the evaluating the thickness of graphene sheets. Functional groups of graphene were determined by FTIR spectroscopy and Raman spectra supported the AFM and FTIR results. Main electrochemical properties of the graphene were studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). In the following, a biosensing application was investigated to determine ascorbic acid (AA) via a non-enzymatic interaction between the graphene and AA. The present graphene-modified BSA (G-BSA) based sensor provided a favorable opportunity for the design of non-enzymatic electrochemical sensors with extraordinary performance via higher some sensing characteristics and simple fabrication.
机译：在这项研究中，我们通过绿色方法合成了石墨烯，其中生产过程中化学材料的消耗量减少并达到了最低值。牛血清白蛋白（BSA）被用作片状石墨烯片的稳定剂。通过紫外可见光谱法证实了官能化石墨烯的合成。将合成的石墨烯的物理和化学性质与常规的氧化石墨烯进行了比较。利用原子力显微镜（AFM）评估石墨烯片的厚度。石墨烯的官能团通过FTIR光谱确定，拉曼光谱支持AFM和FTIR结果。通过循环伏安法（CV）和电化学阻抗谱（EIS）研究了石墨烯的主要电化学性能。在下文中，对生物传感应用进行了研究，以通过石墨烯与AA之间的非酶相互作用确定抗坏血酸（AA）。本发明的基于石墨烯改性的BSA（G-BSA）的传感器通过更高的某些感测特性和简单的制造，为设计具有非凡性能的非酶电化学传感器提供了良好的机会。

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