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首页> 外文期刊>International Journal of Electrochemical Science >Separation and Analysis of Chlorpromazine and Demethylchlorpromazineby Capillary Electrophoresis Coupled with Electrochemiluminescence
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Separation and Analysis of Chlorpromazine and Demethylchlorpromazineby Capillary Electrophoresis Coupled with Electrochemiluminescence

机译：毛细管电泳-电化学发光分离和分离氯丙嗪和去甲基氯丙嗪

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Demethylchlorpromazine (DMCPZ) is one of the main metabolites of chlorpromazine (CPZ). DMCPZhas only one methyl less than CPZ. The structural difference between them is too small to separatethem by conventional capillary electrophoresis (CE).However, proper addition of additives in theseparation buffer can improve their separation performance. The parameters related to CE separationwere carefully studied. The optimized CE conditions were separation buffer of 17.50% iso-propylalcohol aqueous solution (v/v) containing 21mmol/L phosphate (pH 5.3), separation voltage of 13.1kV, sample injection time of 6 s and sample injection voltage of 11.5 kV. Combined withelectrochemiluminescence (CEL) detection technology, a separation and analysis method of CPZ andDMCPZ by improved CE coupled with end-column CEL was established. The limits of detection (3σ)of this method were 8.1×10 -7 mg/mL for CPZ and 8.9×10 -6 mg/mL for DMCPZ. The relative standarddeviations were less than 2.5% for ECL intensity and less than 1.2% for migration time. This methodhad many advantages, such as fast speed, high sensitivity, small sample injection, and free frominterference, and was successfully applied to simultaneously determine CPZ and DMCPZ in wholeurine sample of pet dog.
机译：脱甲基氯丙嗪（DMCPZ）是氯丙嗪（CPZ）的主要代谢产物之一。 DMCPZ仅比CPZ少一个甲基。它们之间的结构差异很小，无法通过常规毛细管电泳（CE）分离。但是，在这些分离缓冲液中适当添加添加剂可以改善其分离性能。仔细研究了与CE分离有关的参数。优化的CE条件为：含有21mmol / L磷酸盐（pH 5.3）的17.50％异丙醇水溶液（v / v）的分离缓冲液，分离电压为13.1kV，进样时间为6 s，进样电压为11.5 kV。结合电化学发光（CEL）检测技术，建立了改进的CE结合端柱CEL分离CPZ和DMCPZ的方法。该方法的检出限（3σ）对CPZ为8.1×10 -7 mg / mL，对DMCPZ为8.9×10 -6 mg / mL。 ECL强度的相对标准偏差小于2.5％，迁移时间的相对标准偏差小于1.2％。该方法具有速度快，灵敏度高，进样量小，无干扰等优点，已成功用于同时测定宠物狗全血样中的CPZ和DMCPZ。

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