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首页> 外文期刊>International Journal of Electrochemical Science >Electrochemical Studies on the Recognition of a Ternary Copper Complex to Single-Stranded DNA and Double-Stranded DNA
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Electrochemical Studies on the Recognition of a Ternary Copper Complex to Single-Stranded DNA and Double-Stranded DNA

机译：电化学研究三元铜配合物对单链DNA和双链DNA的识别

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摘要

The binding actions of a mixed-ligand copper complex, [Cu(bpy)(MBZ)2(H2O)] (bpy = 2, 2- bipyridine, MBZ = p-methylbenzoate) to single-stranded (ss-) DNA and double-stranded (ds-) DNAwere comprehensively studied via electrochemical methods in this paper. Cyclic and differential pulsevoltammetry carried out in homogeneous solution showed that the copper complex underwent differentvariation after interaction with dsDNA and ssDNA, showing the different binding mechanism of thecopper complex to dsDNA and ssDNA. The determination of the electron-transfer rates (Ks) suggestedthat the electro-activities of [Cu(bpy)(MBZ)2(H2O)]-dsDNA and [Cu(bpy)(MBZ)2(H2O)]-ssDNAcomplexes decreased in different extents compared with the free state of the complex. Thethermodynamic binding parameters and kinetic dissociation constants of [Cu(bpy)(MBZ)2(H2O)] withdsDNA and ssDNA were also assessed by surface methods. Based on all the results, an intercalativemode of the copper complex to dsDNA and a coordination mode of the copper complex to ssDNAwere proposed, which was further testified by the emission experiments.
机译：混合配体铜络合物[Cu（bpy）（MBZ）2（H2O）]（bpy = 2、2-联吡啶，MBZ =对甲基苯甲酸酯）对单链（ss-）DNA和双链DNA的结合作用本文通过电化学方法对双链（ds-）DNA进行了全面研究。在均相溶液中进行的循环和差分脉冲伏安法表明，铜配合物在与dsDNA和ssDNA相互作用后经历了不同的变化，表明铜配合物与dsDNA和ssDNA的结合机理不同。电子传递速率（Ks）的确定表明[Cu（bpy）（MBZ）2（H2O）]-dsDNA和[Cu（bpy）（MBZ）2（H2O）]-ssDNA复合物的电活性降低与复合物的自由状态相比，程度不同。还通过表面方法评估了[Cu（bpy）（MBZ）2（H2O）]与dsDNA和ssDNA的热力学结合参数和动力学解离常数。根据所有结果，提出了铜配合物与dsDNA的嵌入方式和铜配合物与ssDNA的配合方式，并通过发射实验进一步证明。

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