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首页> 外文期刊>International Journal of Environment and Bioenergy >Increased Enzymatic Hydrolysis of Cellulose and Hemicellulose due to the Addition of Gluten and Triticale Grist
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Increased Enzymatic Hydrolysis of Cellulose and Hemicellulose due to the Addition of Gluten and Triticale Grist

机译：由于添加了麸质和黑小麦粒，纤维素和半纤维素的酶水解增加

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摘要

The enzymatic activity of cellulases depends very much on their tendency to bind to lignin. Accordingly, crude wheat gluten supplement in mashes has been investigated concerning its effect on the extent of hydrolysis. The reasons for the use of crude gluten were the comparability to triticale grist and the high unspecific binding potential to the different lignin types. Hemp straw and miscanthus straw were steam-pretreated (155 °C, 45 min, no additives) and treated with gluten either during hydrolysis or fermentation. Hydrolysis of hemp straw in the presence of crude gluten achieved up to 50 % higher yields of free glucose and 75 % of xylose after 72 h (miscanthus 55 % more glucose, 45 % more xylose). In further experiments, gluten was added to prehydrolyzed mashes prior to fermentation, but this did not improve the fermentation extents. Simultaneously fermented lignocellulosic and triticale grist mashes were investigated for lignin-protein-ethanol interactions. Most mixtures achieved significantly higher cellulose hydrolysis and fermentation extents. UV-VIS measurements indicate binding of the lignin degradation products.
机译：纤维素酶的酶促活性在很大程度上取决于它们与木质素结合的趋势。因此，已经研究了中的粗小麦面筋补充剂对其水解程度的影响。使用粗面筋的原因是与黑小麦粉的可比性以及与不同木质素类型的高非特异性结合潜力。大麻秸秆和桔梗秸秆经过蒸汽预处理（155°C，45分钟，无添加剂），并在水解或发酵过程中用面筋处理。在72小时后，在粗面筋存在下，大麻秸秆的水解可提高高达50％的游离葡萄糖和75％的木糖产量（芒草木糖多55％，木糖多45％）。在进一步的实验中，在发酵之前将面筋添加到预水解的es中，但这并没有改善发酵程度。研究了同时发酵的木质纤维素和小黑麦麦芽糊精的木质素-蛋白质-乙醇相互作用。大多数混合物实现了明显更高的纤维素水解和发酵程度。 UV-VIS测量表明木质素降解产物的结合。

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