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首页> 外文期刊>International Journal of Electrochemical Science >Laccase Carbon Paste Based Biosensors for Antioxidant Capacity. The Effect of Different Modifiers
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Laccase Carbon Paste Based Biosensors for Antioxidant Capacity. The Effect of Different Modifiers

机译：基于漆酶碳糊的生物传感器具有抗氧化能力。不同修饰符的作用

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The natural antioxidants of food products have great influence on human health. Indeed, the protectiveeffects against cardiovascular diseases, cancer and ageing have been attributed to their polyphenolcontent. In turn, the remarkable antioxidant activity of polyphenols can be easily estimated bypolyphenol oxidases based biosensors. The aim of this work was check the effect of modifiers on theimprovement of biosensors concerning to performance, in order to express the antioxidant activity andtotal polyphenol content of complex natural samples such as coffee beverages. The biosensors wereconstructed by occlusion of crude extract of Pycnoporus sanguineus containing laccase, in differentcarbon paste based composites. The electrochemical properties of the resulting composites, namelyCarbon Paste-Laccase (CPL), CPL modified with Glutaraldeide (CPL-Glu), Bovine Serum Albumin(CPL-BSA), Chitosan (CPL-Ch), Silica (CPL-SiO), Titanium dioxide (CPL-TiO), DNA (CPL-DNA),Carbon Nanotubes (CPL-CN), non-activated Carbon Nanotubes (CPL-nCN) and activated CarbonNanotubes plus DNA (CPL-DNA:CN) were evaluated by voltammetry in different experimentalconditions. In order, the effect of electrolyte composition, pH, scan rate, pulse amplitude, responsetime and concentration range were carefully evaluated, whereas rutin and/or cathecol were employedas standard solutions. The highest sensitivity level and lowest detection limit were obtained for CPL- DNA:CN. In addition, the biosensor demonstrated suitable stability over 10 days and goodrepeatability (RSD < 5%). CPL-DNA:CN was used for the determination of total phenol content ofcoffee samples, expressed as Gallic Acid equivalents (GAEq) and the results were compared withthose from the DPPH and Foliniocalteu spectrophotometric methods. Further, the inherentsensitivity of electroanalytical methods, the amperometric biosensor exhibits some interestingadvantages such as simplicity due to the minimal sample preparation and leading to rapidity ofantioxidant capacity assay.
机译：食品中的天然抗氧化剂对人体健康影响很大。实际上，针对心血管疾病，癌症和衰老的保护作用已经归因于其多酚含量。反过来，可以通过基于多酚氧化酶的生物传感器轻松评估多酚的卓越抗氧化活性。这项工作的目的是检查改性剂对生物传感器性能改善的影响，以表达复杂天然样品（如咖啡饮料）的抗氧化活性和总多酚含量。生物传感器是通过在不同的碳糊基复合材料中封闭含有漆酶的血缘碧萝crude粗提物而构建的。所得复合材料的电化学性能，即碳糊漆酶（CPL），戊二醛修饰的CPL（CPL-Glu），牛血清白蛋白（CPL-BSA），壳聚糖（CPL-Ch），二氧化硅（CPL-SiO），钛通过伏安法分别测定了二氧化碳（CPL-TiO），DNA（CPL-DNA），碳纳米管（CPL-CN），非活性碳纳米管（CPL-nCN）和活性碳纳米管加DNA（CPL-DNA：CN）实验条件。为了仔细地评估电解质组成，pH，扫描速率，脉冲幅度，响应时间和浓度范围的影响，而芦丁和/或乙醇被用作标准溶液。 CPL-DNA：CN获得了最高的灵敏度水平和最低的检测限。此外，该生物传感器在10天内表现出合适的稳定性和良好的重复性（RSD <5％）。 CPL-DNA：CN用于测定咖啡样品中的总酚含量，用没食子酸当量（GAEq）表示，并将结果与​​DPPH和Foliniocalteu分光光度法的结果进行比较。此外，由于电分析方法的固有敏感性，电流型生物传感器表现出一些有趣的优势，例如由于样品制备量最少，并且由于抗氧化剂容量测定的快速性而变得简单。

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