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首页> 外文期刊>International Journal of Electrochemical Science >A Disposable Electrochemical Immunosensor Based on PDDA-functionalized Graphene/Gold Nanoparticles Composites for Detection of Inflammatory Cytokine Interleukin-22
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A Disposable Electrochemical Immunosensor Based on PDDA-functionalized Graphene/Gold Nanoparticles Composites for Detection of Inflammatory Cytokine Interleukin-22

机译：基于PDDA功能化石墨烯/金纳米颗粒复合材料的一次性电化学免疫传感器用于检测炎性细胞因子白介素22。

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摘要

A sandwich-format electrochemical immunoassay strategy was proposed for the single-proteindetection of inflammatory cytokine, Interleukin-22 (IL-22). The capture anti-IL22 (Ab1) and detectionanti-IL22(Ab2) were immobilized onto poly(dimethyldiallylammonium chloride)-functionalizedgraphene/gold nanoparticles nanocomposites (PDDA-G/AuNPs) modified indium tin oxide-coatedglass (ITO) electrodes. When the immunosensor was incubated with sample antigens and biotin- modified Ab2 successively, streptavidin-modified horseradish peroxidase (HRP) was immobilized toproduce the electrochemical signal by using o-phenylenediamine (OPD) and H2O2 as the electrochemical substrates. The linear ranges of IL-22 were from 5 to 5000pg mL with the detectionlimits of 0.5 pg mL (S/N=3), which is lower than that of the conventional ELISA. Experimentalresults demonstrated a ultrasensitive proposed immunosensor and revealed a selective and reproduciblemeasurement of bioactive molecules in clinical diagnosis and therapy.
机译：提出了一种夹心形式的电化学免疫测定策略，用于炎症细胞因子白细胞介素22（IL-22）的单蛋白检测。将捕获抗IL22（Ab1）和检测抗IL22（Ab2）固定在聚（二甲基二烯丙基氯化铵）功能化的石墨烯/金纳米颗粒纳米复合材料（PDDA-G / AuNPs）修饰的氧化铟锡涂层玻璃（ITO）电极上。当免疫传感器与样品抗原和生物素修饰的Ab2连续孵育时，链亲和素修饰的辣根过氧化物酶（HRP）被固定化，以邻苯二胺（OPD）和H2O2为电化学底物产生电化学信号。 IL-22的线性范围为5至5000pg mL，检测限为0.5 pg mL（S / N = 3），低于常规ELISA的检测限。实验结果证明了一种超灵敏的免疫传感器，并揭示了在临床诊断和治疗中对生物活性分子的选择性和可重复性测量。

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