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首页> 外文期刊>International Journal of Electrochemical Science >Covalent Immobilization of Glucose Oxidase onto Electro- synthesized Nanocomposite with PEDOT Derivative for Amperometric Glucose Biosensing
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Covalent Immobilization of Glucose Oxidase onto Electro- synthesized Nanocomposite with PEDOT Derivative for Amperometric Glucose Biosensing

机译：用PEDOT衍生物将葡萄糖氧化酶共价固定在电合成的纳米复合材料上，用于安培葡萄糖生物传感

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摘要

A highly sensitive and specific glucose (Glu) amperometric biosensor was successfully developed bythe covalent immobilization of glucose oxidase (GOx) onto the electro-synthesized ionic liquid (ILs)modified poly(3,4-ethylenedioxylthiophene) derivative poly(hydroxymethylated-3,4-ethylenedioxylthiophene) (PEDOTM) nanocomposite based on multi-walled carbon nanotubesfunctionalized with carboxyl group (MWCNTs-COOH). A highly-stable and conducting PEDOTMwas one-step electro-synthesized in water/ILs mixed system containing BmimPF6 and MWCNTsCOOH,then GOx was covalently immobilized onto the biocompatible ILs modified PEDOTMMWCNTs-COOH with high affinity, which was employed for amperometric biosensing of Glu inhuman urine, human and animal serum samples. IL/PEDOTM-MWCNTs-COOH displayed goodelectrochemical activity, excellent electrochemical stability and high conductivity. The fabricated GOxbiosensor showed pronounced amperometric current toward Glu response in a wide linear range of6.0×10-8～2×10-3 M with a high sensitivity of 89.5 μA M?1 cm?2, rapid response time within 10 s, lowlimit of detection of 0.015 μM, remarkable biocompatibility and bioaffinity, high sensing stability,excellent selectivity and practicality. Satisfactory results reveal that the ILs-PEDOTM-MWCNTsCOOH will provide a promising biosensing platform for the covalent immobilization ofbiomacromolecules and disease diagnostics via the detection of Glu in human and animal serumsamples.
机译：通过将葡萄糖氧化酶（GOx）共价固定在电合成离子液体（ILs）修饰的聚（3,4-乙撑二氧噻吩）衍生物聚（羟甲基化-3,4）上，成功开发了一种高灵敏度和特异性的葡萄糖（Glu）安培生物传感器。 -乙二氧基噻吩（PEDOTM）纳米复合材料，基于用羧基官能化的多壁碳纳米管（MWCNTs-COOH）。在包含BmimPF6和MWCNTsCOOH的水/ IL混合体系中一步一步电合成高度稳定且导电的PEDOTM，然后将GOx以高亲和力共价固定在生物相容的ILs修饰的PEDOTMMWCNTs-COOH上，用于人类对Glu的安培生物传感尿液，人和动物血清样本。 IL / PEDOTM-MWCNTs-COOH具有良好的电化学活性，优异的电化学稳定性和高电导率。制成的GOxbio传感器在6.0×10-8〜2×10-3 M的宽线性范围内显示出明显的Glu响应电流，其高灵敏度为89.5μAM？1 cm？2，响应时间在10 s之内，最低检测限为0.015μM，具有出色的生物相容性和生物亲和力，高传感稳定性，优异的选择性和实用性。令人满意的结果表明，通过检测人和动物血清样品中的Glu，ILs-PEDOTM-MWCNTsCOOH将为生物大分子的共价固定和疾病诊断提供有希望的生物传感平台。

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