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A Novel Glucose Biosensor at Glucose Oxidase Immobilized Graphene and Bismuth Nanocomposite Film Modified Electrode

机译:葡萄糖氧化酶固定的石墨烯和铋纳米复合薄膜修饰电极上的新型葡萄糖生物传感器

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We have prepared graphene-bismuth nanoparticle (Bi) nanocomposite by simple solution basedchemical reduction method and explored its biosensor application for the enzymatic determination ofglucose. The characterization results revealed the successful formation of the composite. Thegraphene-Bi nanocomposite was used for the immobilization of glucose oxidase (GOx). Directelectron transfer of GOx was observed with well-defined redox peaks at the formal potential of 0.427V. The amount of electroactive GOx (袚) and electron transfer rate constant (ks) were calculated to be-10 -2 -1 5.4810 mol cm and 5.57 s , respectively. An amperometric glucose biosensor has been fabricatedwhich detects glucose in a wide linear range from 1 mM to 12 mM with high sensitivity (2.243-1 -2 AmM cm ). Practical feasibility of the biosensor is explored in human blood serum sample whichare revealed the excellent practicality of the biosensor. Moreover, the sensor exhibited appreciablestability, repeatability and reproducibility.
机译:我们通过简单的基于溶液的化学还原方法制备了石墨烯-铋纳米粒子(Bi)纳米复合材料,并探索了其在生物传感器中的应用。表征结果揭示了复合物的成功形成。石墨烯-Bi纳米复合材料用于固定葡萄糖氧化酶(GOx)。观察到GOx的直接电子转移,在0.427V的形式电势下具有明确定义的氧化还原峰。计算出的电活性GOx的量(袚)和电子传递速率常数(ks)分别为-10 -2 -1 5.4810 mol cm和5.57 s。已经制造出安培型葡萄糖生物传感器,其以高灵敏度(2.243-1 -2 AmM cm)检测从1 mM到12 mM的宽线性范围内的葡萄糖。在人血清样品中探索了该生物传感器的实际可行性,这揭示了该生物传感器的优异实用性。此外,该传感器表现出明显的稳定性,可重复性和可再现性。

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