A Sensitive Electrochemical Method for Detection of Histone Deacetylase with the Signal Amplification of Ferrocene-Capped Gold Nanoparticle/Streptavidin Conjugates

Yanping Gao; Ting Sun; Chengye Yang; Binbin Zhou

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A Sensitive Electrochemical Method for Detection of Histone Deacetylase with the Signal Amplification of Ferrocene-Capped Gold Nanoparticle/Streptavidin Conjugates

机译：二茂铁加帽的金纳米粒子/链霉亲和素结合物的信号放大检测组蛋白脱乙酰酶的灵敏电化学方法。

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This work reported the amplified voltammetric detection of histone deacetylase (HDAC) usingferrocene (Fc)-capped gold nanoparticle/streptavidin (SA-AuNPs) conjugates. The acetylated peptidesubstrates immobilized on the gold electrodes were deacetylated by HDAC, resulting in the appearanceof free amine groups on electrode surface. The exposed primary amines were then derivatized withbiotin for the attachment of Fc-capped SA-AuNPs conjugates. The voltammetric responses were foundto be proportional to the HDAC concentrations ranging from 0.1 to 30 nM with a detection limit of 20pM.

机译：这项工作报道了使用二茂铁（Fc）封端的金纳米颗粒/链霉亲和素（SA-AuNPs）缀合物对组蛋白脱乙酰基酶（HDAC）的伏安检测。固定在金电极上的乙酰化肽底物通过HDAC脱乙酰化，导致电极表面出现游离胺基。然后将暴露的伯胺用生物素衍生化以附着Fc封端的SA-AuNPs共轭物。发现伏安响应与HDAC浓度成正比，范围从0.1到30 nM，检测极限为20pM。

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《International Journal of Electrochemical Science》 |2015年第4期|共6页
作者
Yanping Gao; Ting Sun; Chengye Yang; Binbin Zhou;
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中图分类化学;
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A Sensitive Electrochemical Method for Detection of Histone Deacetylase with the Signal Amplification of Ferrocene-Capped Gold Nanoparticle/Streptavidin Conjugates

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