• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Electrochemical Science >Detection of Interferon-Gamma for Latent Tuberculosis Diagnosis Using an Immunosensor Based on CdS Quantum Dots Coupled to Magnetic Beads as Labels
【24h】

Detection of Interferon-Gamma for Latent Tuberculosis Diagnosis Using an Immunosensor Based on CdS Quantum Dots Coupled to Magnetic Beads as Labels

机译：基于CdS量子点结合磁珠的免疫传感器检测γ-干扰素对潜伏性肺结核的诊断

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We describe a rapid, sensitive, and enzyme-free method to detect interferon-gamma (IFN-), which isassociated with susceptibility to tuberculosis. A novel immunosensor using CdS quantum dots (QDs)coupled to magnetic beads (MB) as electrochemical labels has been developed for detection of IFN-.This sandwich-type sensor is fabricated on a glassy carbon electrode (GCE) covered with a well- ordered gold nanoparticles (AuNPs) monolayer, which offered a solid support to immobilize captureanti-human IFN- antibodies (Ab1) efficiently. Then MB-QDs conjugated with detection anti-humanIFN-antibodies (Ab2) were attached onto the AuNPs surface through a subsequent 渟andwich immunoreaction. Square wave anodic stripping voltammetry (SWASV) was carried out to quantify themetal cadmium, which indirectly reflected the amount of the analyte. Under optimum conditions, theelectrochemical signal showed a linear relationship with the logarithm of IFN- concentration, ranging-1 -1 -1 from 1 pg mL to 500 pg mL , and the detection limit was 0.34 pg mL . The immunosensor showedhigh sensitivity, satisfactory reproducibility and reproducibility, and could be used for the detection ofreal sample, which provided a potential tool for latent tuberculosis diagnosis.
机译：我们描述了一种快速，灵敏，无酶的方法来检测干扰素-γ（IFN-），它与结核病易感性有关。已开发出一种新颖的免疫传感器，将CdS量子点（QD）耦合到磁珠（MB）作为电化学标记，用于检测IFN-。该三明治型传感器在覆盖有序的玻碳电极（GCE）上制造金纳米颗粒（AuNPs）单层，为有效固定捕获抗人IFN-抗体（Ab1）提供了坚实的支持。然后，通过随后的夹心免疫反应将与检测抗人IFN-抗体（Ab2）缀合的MB-QD连接到AuNPs表面。进行方波阳极溶出伏安法（SWASV）定量分析金属镉，该金属镉间接反映了分析物的量。在最佳条件下，电化学信号与干扰素浓度的对数呈线性关系，范围为1 pg -1至500 pg mL -1 -1 -1，检出限为0.34 pg mL。该免疫传感器灵敏度高，重复性和重复性令人满意，可用于实际样品的检测，为潜在的结核病诊断提供了潜在的工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号